Partial Purification and Characterization of Acid and Neutral α-Glucosidases from Preclimacteric Banana Pulp Tissues
スポンサーリンク
概要
- 論文の詳細を見る
An acid α-glucosidase (AAG) with an optimum pH of 4.5 and two isoforms of neutral α-glucosidase (NAG I and II) with an optimum pH of 6.5 were partially purified from preclimacteric banana pulp tissues by monitoring the 4-methylumbelliferyl α-D-glucoside (4MUαG) hydrolyzing activity. The molecular weights of the AAG and the two NAG were 70,000 and 42,000,respectively, by gel filtration. By kinetic studies, the AAG was found to be a typical maltase that required substrates such as maltose, maltotriose, maltotetraose, and maltopentaose rather than soluble starch. On the other hand, the two NAGs preferred 4MUαG to maltose as substrate and their maltase activities were about 50 times lower than that of the AAG. The NAGs, as well as the AAG, did not hydrolyze isomaltose, trehalose, sucrose, or glycogen at all. Sucrose was a competitive inhibitor of the AAG but not NAGs toward 4MUαG. Glucose and maltose were also competitive inhibitors of both AAG and NAGs.
- 社団法人日本農芸化学会の論文
- 1992-12-23
著者
-
Konishi Yuko
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
-
Konishi Y
Kirin Brewery Co. Ltd. Gunma Jpn
-
Konishi Yotaro
Department Of Food And Nutrition Faculty Of Human Life Science Osaka City University
-
Nakatani N
Osaka City Univ. Osaka Jpn
-
Nakatani Nobuji
Department Of Food And Nutrition Faculty Of Human Life Science Osaka City University
-
KITAZATO Sachiko
Department of Food and Nutrition, Faculty of Science of Living, Osaka City University
-
Kitazato Sachiko
Department Of Food And Nutrition Faculty Of Science Of Living Osaka City University:(present Address
-
Konishi Yotaro
Department of Applied Chemistry, Faculty of Engineering, Nagasaki University
関連論文
- Chemopreventive Effects of Coumaperine from Pepper on the Initiation Stage of Chemical Hepatocarcinogenesis in the Rat
- Lack of Inhibitory Effects of Coumaperine from Pepper on the Promotion Stage of Chemical Hepatocarcinogenesis in the Rat
- Carbohydrate-Dependent Hemolytic Activity of the Conjugate Composed of a C-Type Lectin, CEL-I, and an Amphiphilic α-Helical Peptide, 4_3-βAla_2
- Preparation of A Ca^〈2+〉-Dependent Lectin-Basic Amphiphilic Peptide Conjugate, and Its Interaction with Biomembrane
- Identification of the Modified Structure of Arginine Residues in Proteins with 3-Deoxyglucosone, a Maillard Reaction Intermediate
- Various Cooking Methods and the Flavonoid Content in Onion
- β-Glucosidase Activity in the Rat Small Intestine toward Quercetin Monoglucosides
- Antioxidative Activity of Arbutin in a Solution and Liposomal Suspension
- Effects of Bay m 1099, an α-Glucosidase Inhibitor, on Starch Metabolism in Germinating Wheat Seeds
- Report on the preparation of deuterium-labelled aconitine and mesaconitine and their application to the analysis of these alkaloids from body fluids as internal standard
- Spasmolytic Activity of Aurapten Analogs
- Epoxyaurapten and Marmin from Juice Oil in Hassaku (Citrus hassaku) and the Spasmolytic Activity of 7-Geranyloxycoumarin-related Compounds(Organic Chemistry)
- 7-Geranyloxycoumarin from Juice Oil of Hassaku (Citrus hassaku) and Antimicrobial Effects of Related Coumarins(Food & Nutrition)
- Novel Imidazolone Compound Formed by the Advanced Maillard Reaction of 3-Deoxyglucosone and Arginine Residues in Proteins
- Globulin and Albumin-2 Associated with Protein Bodies in Amaranthus cruentus Seeds
- The Effects of Endosperm Mutations and Environmental Temperature during Development on the Distribution of Molecular Weight of Amylose in Rice Endosperms(Biological Chemistry)
- Spasmolytic Activity of Geranyloxycoumarin-related Compounds
- Isolation and Characterization of Globulin from Seeds of Amaranthus hypochondriacus L.
- Characterization of Starch Granules from Waxy, Nonwaxy, and Hybrid Seeds of Amaranthus hypochondriacus L.
- Fractionation of Rat Liver Glycogen Particles on a Sephacryl S-1000 Gel Column and Their Structural Properties
- Antioxidative Effect of the Constituents of Rosemary (Rosmarinus officinalis L.) and Their Derivatives
- Structure of a New Antioxidative Phenolic Diterpene Isolated from Rosemary (Rosmarinus officinalis L.)
- Glycogenosomes in Hepatocyte of Diploid and Triploid Masu Salmon(Oncorhynchus masou), Mimicking Cytology of Glycogen Storage Disease Type II(Biological Chemistry)
- Identification of Kaempferol from the Leaves of Diospyros kaki and its Antimicrobial Activity against Streptococcus mutans
- Effects of Bay m 1099, an α-Glucosidase Inhibitor, on Starch Degradation in Germinating Mung Beans
- Characterization of Photosynthetic Glycogen and α-Glucosidase from Thermophilic Cyanobacteria, Lyngbya-Phormidium-Plectonema Group B
- Glycogen as Primordial Carbon Reserve and α-Glucosidase in the Genera Lyngbya-Phormidium-Plectonema, Thermophilic Cyanobacteria
- Partial Purification and Characterization of Acid and Neutral α-Glucosidases from Preclimacteric Banana Pulp Tissues
- Extraction of Soluble and Bound α-Glucosidases from Banana Pulp(Food & Nutrition)
- Polymorphism of Acid and Neutral α-Glucosidases in Banana Pulp : Changes in Apparent pIs and Affinity to Con A of the Enzymes during Ripening(Food & Nutrition)
- Effect of Moisture Content on the Expansion Volume of Popped Amaranth Seeds by Hot Air and Superheated Steam Using a Fluidized Bed System
- FORMATION OF GLYCOGENOSOMES IN RAT LIVER INDUCED BY INJECTION OF ACARBOSE, AN α-GLUCOSIDASE INHIBITOR
- LC-MS Analysis and Structural Determination of New Amides from Javanese Long Pepper (Piper retrofractum)
- Microbial Transformation of (±)-Epoxyaurpten by Pseudomonas aeruginosa
- Transmission Electron Microscopy of Protein Bodies in the Amaranthus cruentus Seed Embryo
- Pungent Alkamides from Spilanthes acmella L. var. oleracea Clarke
- Amaranth Globulin as a Heat-stable Emulsifying Agent(Food & Nutrition)
- Structure of New Deodorant Biphenyl Compounds from Thyme (Thymus vulgaris L.) and Their Activity Against Methyl Mercaptan(Organic Chemistry)
- Extraction of Two Albumin Fractions from Amaranth Grains : Comparison of Some Physicochemical Properties and the Putative Localization in the Grains(Food & Nutrition)
- Antioxidative Activity of Flavonoids from Thyme (Thymus vulgaris L.)(Organic Chemistry)
- A Pitfall in Determining the Globulin/Albumin Ratio in Amaranth Grains.
- A New Antioxidative Glucoside Isolated from Oregano (Origanum vulgare L.)
- Structure of Rosmanol, A New Antioxidant from Rosemary (Rosmarinus officinalis L.)
- Isobutyl Amides from Pepper (Piper nigrum L.)
- Structure of a New Antioxidative Phenolic Acid from Oregano (Origanum vulgare L.)
- Two Antioxidative Diterpenes from Rosemary(Rosrnarinus officinalis L.) and a Revised Structure for Rosmanol
- A New Diterpene Lactone, Rosmadial, from Rosemary (Rosrnarinus officinalis L.)
- Urinary Electrolytes and Trace Elements in Stroke-prone SHR