Extraction of Two Albumin Fractions from Amaranth Grains : Comparison of Some Physicochemical Properties and the Putative Localization in the Grains(Food & Nutrition)
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概要
- 論文の詳細を見る
Two albumin fractions were extracted from defatted flours of seeds of Amaranthus hypochondriacus and A. cruentus. One fraction (Alb-1) was extracted with 0.5 M NaCl or water when used as the first solvent. Another fraction (Alb-2) was extracted with water after removing Alb-1 and globulin. Apparent pIs of Alb-1 and Alb-2 were 4.5-7.5 and 5-6, respectively. Alb-2 was precipitated by various metal ions (1 mM), while Alb-1 was not. Alb-2 contained four major components (M_r=56, 36, 26, 22 kD), although Alb-1 had no particular major proteins. Pronase treatment of amaranth flour before protein extraction decomposed Alb-1, but not Alb-2, as seen by SDS-PAGE analysis. The findings that Alb-2 was protected from the Pronase treatment and it could not be extracted until Alb-1 and globulin were removed suggests that Alb-2 might be associated with protein bodies [Irving et al., J. Food Sci., 46, 1170 (1981)].
- 社団法人日本農芸化学会の論文
- 1991-11-23
著者
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Konishi Y
Osaka City Univ. Osaka Jpn
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Konishi Yotaro
Department Of Food And Nutrition Faculty Of Human Life Science Osaka City University
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Nakatani N
Laboratory Of Food Chemistry Faculty Of Science Of Living Osaka City University
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Nakatani Nobuji
Department Of Food And Nutrition Faculty Of Human Life Science Osaka City University
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HORIKAWA Kazuko
Department of Food and Nutrition, Faculty of Science of Living, Osaka City University
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OKU Yoko
Department of Food and Nutrition, Faculty of Science of Living, Osaka City University
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AZUMAYA Junko
Department of Food and Nutrition, Faculty of Science of Living, Osaka City University
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Oku Yoko
Department Of Food And Nutrition Faculty Of Science Of Living Osaka City University
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Azumaya Junko
Department Of Food And Nutrition Faculty Of Science Of Living Osaka City University
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Horikawa Kazuko
Department Of Food And Nutrition Faculty Of Science Of Living Osaka City University
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Konishi Yotaro
Department of Applied Chemistry, Faculty of Engineering, Nagasaki University
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