Measurement of 3-Hydroxyanthranilic Acid and Anthranilic Acid in Urine by High-performance Liquid Chromatography(Analytical Chemistry)
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概要
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Measurements of 3-hydroxyanthranilic acid and anthranilic acid in urine are described. The urine adjusted to pH3.0 was saturated with NaCl, and 3-hydroxyanthranilic acid and anthranilic acid were extracted with diethyl ether. The method for 3-hydroxyanthranilic acid measurement employed a Shim-pack CLC-ODS (150mm×6.0mm i.d.) column eluted with 100m_M potassium dihydrogenphosphate (pH 4.5)-acetonitrile (9:1, v/v) at a flow rate of 1.5 ml/min. The applied volage was set at +300 mV vs. Ag/AgCl, the detection limit being 0.2pmol (30.62pg) at a signal-to-noise ratio of 5:1. The method for anthranilic acid measurement employed a Chemcosorb 5-ODS-H (150 mm×4.6mm i.d.) column eluted with 80 m_M potassium dihydrogenphosphate (pH adjusted to 3.0 by phosphoric acid)-acetonitrile (65:35, v/v) at a flow rate of 1.0 ml/min, with estimation at an excitation wavelength of 340 nm and an emission wavelength of 410nm. The detection limit was 0.3pmol (41.13pg) at a signal-to-noise ratio of 5:1. These techniques were applied to the analyses of riboflavin-deficient rat urine. The total analysis times for 3-hydroxyanthranilic acid and anthranilic acid were ca. 12min and 7min, respectively.
- 社団法人日本農芸化学会の論文
- 1991-01-23
著者
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SHIBATA Katsumi
Department of Food Science and Nutrition, School of Human Cultures, The University of Shiga Prefectu
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Shibata Katsumi
Department Of Food Science And Nutrition School Of Human Cultures The University Of Shiga Prefecture
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ONODERA Michiko
Department of Human Health Science, Faculty of Human Sciences, Osaka International University for Wo
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Onodera Michiko
Department Of Food Science And Nutrition Teikoku Women's University
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