High-performance Liquid Chromatographic Measurement of Nicotinamide N-Oxide in Urine after Extracting with Chloroform(Analytical Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
The measurement of nicotinamide N-oxide by high-performance liquid chromatography is described. The method used a Chemcosorb 5-ODS-H (150×4.5mm i.d., particle size 5μm) column eluted with 10mM potassium dihydrogenphosphate (pH was adjusted to 3.0 by adding concentrated phosphoric acid) containing 0.1 g/l of sodium 1-octanesulfonate-methanol (100:4, v/v) at a flow-rate of 1.0 mil min. The UV detector was set at 260 nm. The detection limit for nicotinamide N-oxide was 2 pmol (276 pg) at a signal-to-noise ratio of 5:1. Under these conditions, nicotinamide N-oxide was eluted at about 3.2 min. This technique was used to analyze rat urine following effective clean-up steps with chloroform, the total analysis time being about 13 min.
- 社団法人日本農芸化学会の論文
- 1989-05-23
著者
-
SHIBATA Katsumi
Department of Food Science and Nutrition, School of Human Cultures, The University of Shiga Prefectu
-
Shibata Katsumi
Department Of Food Science And Nutrition School Of Human Cultures The University Of Shiga Prefecture
関連論文
- Effect of Fasting on the Urinary Excretion of Water-Soluble Vitamins in Humans and Rats
- Fluorometric determination of 2-oxoadipic acid, a common metabolite of tryptophan and lysine, by high-performance liquid chromatography with pre-chemical derivatization
- Fluorometric Determination of 2-Oxoadipic Acid, a Common Metabolite of Tryptophan and Lysine, by High-Performance Liquid Chromatography with Pre-Chemical Derivatization
- Effects of Dietary 6-Aminonicotinamide, an Antagonist of Nicotinamide, on the Metabolism of Tryptophan to Nicotinamide in Rats
- High-performance Liquid Chromatographic Measurement of Nicotinamide N-Oxide in Urine after Extracting with Chloroform(Analytical Chemistry)
- Effects of High-voltage Electric Field Treatment on the Water Activity of Bread
- Urinary Excretion of Nicotinamide and Its Metabolites in Alloxandiabetic Rats Fed on a Niacin-free Diet(Food & Nutrition)
- Effects of Ethanol Feeding and Growth on the Tryptophan-niacin Metabolism in Rats(Food & Nutrition)
- Urinary Excretory Ratio of Anthranilic Acid/Kynurenic Acid as an Index of the Tolerable Amount of Tryptophan
- Effects of Peroxidation Products of Linoleic Acid on Tryptophan-nicotinamide Metabolism in Rats
- Effect of Lipid Peroxidation Products on the Catabolic Fate of Nicotinamide in Rats(Food & Nutrition)
- Relative Activity of N-(β-D-Glucopyranosyl)nicotinic Acid to Nicotinic Acid as a Niacin Nutrient in Rats and in Lactobacillus plantarum ATCC 8014
- End Product Inhibition of the Activity of Nicotinamide Phosphoribosyltransferase from Various Tissues of Rats by NAD(Biological Chemistry)
- Comparison of Tryptophan-nicotinamide Metabolism between Male and Female Rats
- Efficiency of Dietary 3-Acetylpyridine, Pyridine-3-aldehyde, Pyridine-3-methanol, and β-Picoline as Niacin in Rats
- Comparison of the Nicotinamide Catabolism among Rat Strains
- Age-Related Alterations of B-Group Vitamin Contents in Urine, Blood and Liver from Rats
- Nutritional Efficiency of Nicotinamide N-Oxide and N'-Methylnicotinamide as Niacin in Rats(Food & Nutrition)
- Changes in Nicotinamide Metabolism by One Amino Acid Deficiency. (II) Isoleucine-, Phenylalanine-, Valine-, Arginine-, or Histidine-free Diet
- Changes in Nicotinamide Metabolism by One Amino Acid Deficiency. (I) Threonine-, Tryptophan-, Aspartic Acid-, Lysine-, Leucine-, or Methionine-free Diet
- Effects of Protein-Free Diet Feeding or Starving on the Excretion Ratio of (N^1-Methyl-2-pyridone-5-carboxamide + N^1-Methyl-4-pyridone-3-carboxamide)/N^1-Methylnicotinamide(Food & Nutrition)
- Effects of UVA Irradiation on the Concentration of Folate in Human Blood
- Tryptophan-Niacin Metabolism in Liver Cirrhosis Rat Caused by Carbon Tetrachloride
- The Formation of Nicotinamide by Administration of Di(2-ethylhexyl)phthalate Does Not Cause Growth Retardation in Young Rats in Contrast with Excess Exogenous Administration of Nicotinamide
- Comparison of Tryptophan-Niacin Conversion in Rats Fed with a Nicotinic Acid-free Diet Containing Egg White, Egg White Proteolysate, or Mixtures of Amino Acids(Food & Nutrition)
- Consideration of Diurnal Variations in Human Blood NAD and NADP Concentrations
- Urinary Water-Soluble Vitamins and Their Metabolite Contents as Nutritional Markers for Evaluating Vitamin Intakes in Young Japanese Women
- Effect of Pyridoxal on the Urinary Excretion of Nicotinamide Metabolites in Rats(Biological Chemistry)
- Effect of Prednisolone on the Urinary Excretion of Nicotinamide and Its Metabolites in Rats Fed with a Niacin-free Diet(Food & Nutrition)
- High-performance Liquid Chromatographic Measurement of Tryptophan in Blood, Tissues, Urine, and Foodstuffs with Electrochemical and Fluorometric Detections(Analytical Chemistry)
- Effects of Dietary Pyrazinamide on the Growth of Weanling Rats Fed with a Nicotinic Acid-free and Tryptophan-limited Diet(Food & Nutrition)
- Relationship between Urinary Excretion of Kynurenic Acid and Protein Intake in Rats(Food & Nutrition)
- Measurement of 3-Hydroxyanthranilic Acid and Anthranilic Acid in Urine by High-performance Liquid Chromatography(Analytical Chemistry)
- Comparison between the Metabolic Fate of Physiological Amount of Dietary Nicotinic Acid and Nicotinamide in Rats(Food & Nutrition)
- Serotonin Levels in Blood and Small Intestine as Influenced by Nicotinic Acid and Quinolinic Acid(Food & Nutrition)
- Fates of N'-Methylnicotinamide and Nicotinamide N-Oxide in Mice(Food & Nutrition)
- Effects of Pyrazinamide on Tryptophan-Niacin Conversion in Rats(Biological Chemistry)
- Effects of Three Kinds of Dietary Nitrogen Sources on the Metabolic Fate of Tryptophan(Food & Nutrition)
- Effects of Thyroxine on the Conversion Ratio of Tryptophan to Nicotinamide in Rats
- Effects of Sex Hormones on the Metabolism of Tryptophan to Niacin and to Serotonin in Male Rats
- Course of the Urinary Excretion of Nicotinic Acid and Its Related Compounds Following the Injection of Optimal Amount of Nicotinic Acid for NAD Synthesis(Biological Chemistry)
- Increased Conversion Ratio of Tryptophan to Niacin by the Administration of Clofibrate, a Hypolipidemic Drug, to Rats
- In Vivo Inhibition of Kynurenine Aminotransferase Activity by Isonicotinic Acid Hydrazide in Rats
- High-Fat Diet Lowers the Nutritional Status Indicators of Pantothenic Acid in Weaning Rats
- Comparison of the Nicotinamide Catabolism among Rat Strains
- Effects of Progesterone and Estrone on the Conversion of Tryptophan to Nicotinamide in Rats
- Effects of Feeding Tryptophan-limiting Diets on the Conversion Ratio of Tryptophan to Niacin in Rats
- Increased Conversion Ratio of Tryptophan to Niacin in Severe Food Restriction
- Effects of Dietary Pyrazinamide on the Metabolism of Tryptophan to Niacin in Streptozotocin-diabetic Rats
- Fluorometric Determination of 2-Oxoadipic Acid, a Common Metabolite of Tryptophan and Lysine, by High-Performance Liquid Chromatography with Pre-Chemical Derivatization
- Vitamin Contents in Rat Milk and Effects of Dietary Vitamin Intakes of Dams on the Vitamin Contents in Their Milk
- Change in the Ratio of N^1-Methyl-2-pyridone-5-carboxamide to N^1-Methyl-4-pyridone-3-carboxamide Excretion Following a Large Amount of N^1-Methylnicotinamide Injection in Rats(Food & Nutrition)
- Effects of Gradually Increasing Levels of Nicotinamide in a Niacin-free and Tryptophan-limited Diet on the Blood NAD levels and the Urinary Excretion of Nicotinamide Metabolites in Rats(Food & Nutrition)
- Inhibition of N^1-Methylnicotinamide Oxidase Activity by a Large Amount of N^1-Methylnicotinamide Injected into Rats(Food & Nutrition)
- Effect of Dietary Soy Protein Isolate Level on the Ratio of N^1-Methyl-2-pyridone-5-carboxamide plus N^1-Methyl-4-pyridone-3-carboxamide to N^1-Methylnicotinamide Excretion in Rats(Food & Nutrition)
- Inhibition of N^1-Methylnicotinamide Oxidase Activity by a Large Nicotinamide Injection into Rats(Biological Chemistry)
- Effect of the Addition of Free Tryptophan to a 20% Casein Diet on the Ratio of N^1-Methyl-2-pyridone-5-carboxamide plus N^1-Methyl-4-pyridone-3-carboxamide to N^1-Methylnicotinamide Excretion(Food & Nutrition)
- Effects of Vitamin B_6 Deficiency on the Conversion Ratio of Tryptophan to Niacin
- High-performance liquid chromatographic measurement of urocanic acid isomers and their ratios in naturally light-exposed skin and naturally shielded skin
- Effects of Exercise on the Metabolism of NAD in Rats
- Fluorometric determination of pantothenic acid in human urine by isocratic reversed-phase ion-pair high-performance liquid chromatography with post-column derivatization
- Fate of Nicotinamide Differs Due to an Intake of Nicotinamide
- Effects of UVA Irradiation on the Concentration of Folate in Human Blood
- The Formation of Nicotinamide by Administration of Di (2-ethylhexyl) phthalate Does Not Cause Growth Retardation in Young Rats in Contrast with Excess Exogenous Administration of Nicotinamide
- Effects of Dietary Protein Levels on the Enzyme Activities Involved in Tryptophan-Niacin Metabolism in Rats(Food & Nutrition)
- Effect of Dietary Paraquat on the Enzyme Activities Involved in Tryptophan-Niacin Metabolism in Rats(Biological Chemistry)
- Simultaneous High-performance Liquid Chromatographic Measurement of Xanthurenic Acid and 3-Hydroxyanthranilic Acid in Urine
- Changes in the Conversion Rate of Tryptophan-nicotinamide According to Dietary Fat and Protein Levels
- Effects of Adrenalin on the Conversion Ratio of Tryptophan to Niacin in Rats
- Effect of Adding Branched-chain Amino Acids to a Nicotinic Acid-free, Low-protein Diet on the Conversion Ratio of Tryptophan to Nicotinamide in Rats
- Change of Tryptophan-Niacin Metabolism in D-Galactosamine-Induced Liver Injury in Rat
- Difference in Tryptophan-nicotinamide Conversion According to Dietary Nitrogen Sources ; Casein, Casein Hydrolysate, and Mixtures of Amino Acids
- Effect of Adding the Limiting Amino Acids to an Amino Acid Diet Simulating Rice Protein on the Conversion of Tryptophan to Nicotinamide in Rats
- Conversion Ratio of Tryptophan to Niacin in Rats Fed a Vitamin B1-Free Diet
- The Urinary Excretory Ratio of Nicotinamide Catabolites Was Associated with the Conversion Ratio of Tryptophan to Nicotinamide in Growing Rats Fed a Niacin-Free 20% Casein Diet
- Effect of Adding Methionine and Threonine to a Protein-free Diet on the Metabolism of Nicotinamide
- Urinary Excretion of Water-Soluble Vitamins Increases in Streptozotocin-Induced Diabetic Rats without Decreases in Liver or Blood Vitamin Content
- Increase in the Urinary Excretion of Nicotinamide and Its Metabolites in Rats Administered 3-Acetylpyridine, an Antagonist of Niacin
- Conversion Ratio of Tryptophan to Niacin in Rats Fed with a Nicotinic Acid-free, Tryptophan-limiting Diet
- The Body Vitamin B1 Levels of Rats Fed a Diet Containing the Minimum Requirement of Vitamin B1 Is Reduced by Exercise
- D-Pantethine Has Vitamin Activity Equivalent to D-Pantothenic Acids for Recovering from a Deficiency of D-Pantothenic Acid in Rats
- Urinary Excretion Levels of Water-Soluble Vitamins in Pregnant and Lactating Women in Japan
- Enzymes That Control the Conversion of L-Tryptophan-nicotinamide and the Urinary Excretion Ratio (N1-Methyl-2-pyridone-5-carboxamide + N1-Methyl-4-pyridone-3-carboxamide)/N1-Methylnicotinamide in Mice
- Dietary Reference Intakes for Japanese 2010: Water-Soluble Vitamins
- Reparative Effect of Nicotinamide and Nicotinic Acid on the Aggravation of Tryptophan-nicotinamide Metabolism Caused by 6-Aminonicotinamide
- Increased Conversion of Tryptophan to Nicotinamide in Rats by Dietary Valproate