Progestin Receptors in Testes from Various Animal Species

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Using a synthetic progestin, R5020 (17α, 21-dimethyl-19-nor-4, 9-pregnadiene-3, 20-dione), we investigated the presence of progestin receptor (s) in testicular cytosol from various animal species including humans. After incubation of cytosol from adult chickens with [^3H] R5020 in the presence or absence of various unlabeled steroids, specific progestin binding was found in the 8 S region with an equilibrium dissociation constant of 2 nM and a binding capacity of 75 fmoles/mg protein. Specific progestin binding was also found in cytosol from immature chickens sedimenting in the 4S region with a dissociation constant of 61 nM and a binding capacity of 1 pmole/mg protein. Although 5S binding was found in the cytosols from humans, a monkey and rats, the binding specificity was low. These results clearly indicate that the testicular cytosols from chickens contain progestin receptor (s), whereas those from the other species do not.
東海大学の論文