The Uptake of Progesterone by the Brain Tissues of Male and Female Rats in vivo and Its Relationship to the Cytoplasmic Progesterone-binding Component

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The uptake of ^3H-progesterone by the brain tissues of gonadectomized male and female rats following an i. v. injection of radiolabeled hormone was studied. In females, ^3H-progesterone concentration was highest in the median eminence, followed by the posterior through anterior hypothalamus to the cerebral cortex. In addition, the median eminence/cortex ratio of radioactivity increased almost linearly with time after the injection. In males, radioactive progesterone concentration in all the hypothalamic regions exceeded that in the cerebral cortex. Among the four basal brain tissues, the median eminence showed the highest tissue/cortex ratio of radioactivity. The progesterone-binding activity of the female rat hypothalamic 107,000xg supernatant was examined in vitro by Sephadex G-200 column analysis. Protein components in the cytosol from the median eminence with high-affinity limitedcapacity progesterone-binding activity were identified. It appears that the preferential uptake of progesterone by the median eminence of the female rat hypothalamus is correlated with the protein components which have high-affinity limited-capacity progesterone-binding activity in the tissue.
東海大学の論文