Further Evidence of the Presence of Progesterone-Binding Proteins in Female Rat Hypothalamus

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Utilizing techniques of Sephadex G-200 column chromatography, sucrose density gradient ultracentrifugation and dextran-coated charcoal adsorption, the characterization of progesterone-binding proteins was attempted in cytosols obtained from the medial basal hypothalamus including the median eminence, the remaining hypothalamus and the cerebral cortex of ovariectomized estrogen-primed rats. After incubation of the medial basal hypothalamic cytosol with 3H-progesterone in the absence or in the presence of various unlabeled steroids, a specific progesteronebinding protein was found in the 7 S region with its binding capacity of -2×10-14moles per, mg protein and dissociation constant of -6×10-9 M. No specific binding was, however, found in the remaining hypothalamus and the cerebral cortex.
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