Mutation Analysis of the p53 Tumor Suppressor Gene using Paraffin-embedded Specimens of Human Transitional Cell Carcinomas by the Direct Sequencing Method
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概要
- 論文の詳細を見る
A very small quantity of DNA was extracted from paraffin sections of 25 bladder and 2 ureteral cancer cases, and exons 5-8 of the p53 gene were amplified by the polymerase chain reaction. Mutations were detected by direct sequencing, and their relationships to clinicopathological factors were assessed. Point mutations of the p53 gene were observed in 2 of 27 specimens of transitional cell carcinoma. One was a novel nonsense mutation. The prognosis of patients with p53 gene mutations was poorer than those of patients without mutations. Analysis of the data showed that assessment of the potential malignancy of bladder cancer, combined with a conventional pathological diagnosis, allows a more precise prognosis. Direct sequencing of tissue specimens enables swift prognostic evaluation and prompt decisions concerning treatment.
- 東海大学の論文
著者
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MASUDA Aiichiro
Department of Oiso Hospital, Tokai University
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KAWAMURA NOBUO
Department of Urology, Tokai University School of Medicine
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Masuda Aiichiro
東海大学 医学部泌尿器科学
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Masuda Aiichiro
Department Of Oiso Hospital Tokai University
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Masuda Aiichiro
Department Of Urology Tokai University School Of Medicine Tokyo Hospital
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Masuda Aiichiro
Department Of Urology Tokai University School Of Medicine
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Kawamura Nobuo
東海大学 泌尿器科
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Kawamura Nobuo
Department Of Urology School Of Medicine Tokai University
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Kawamura Nobuo
Department Of Urology Tokai University School Of Medicine
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Kawamura N
Department Of Urology Tokai University School Of Medicine
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KIKUCHI Yara
Department of Genetic Information, Division of Molecular Life Science, Tokai University School of Me
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Kikuchi Yara
Department Of Genetic Information Division Of Molecular Life Science Tokai University School Of Medi
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Kawamura Nobuo
Departments Of' Urology And Surgery Tokai University School Of Medicine
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