ジエチルニトロサミンと2-アセチルアミノフルオレン投与ハムスターからの肝臓卵形細胞の分離
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概要
- 論文の詳細を見る
最近, 肝癌誘発物質を投与後Clonorchis sinensisを感染させることによってハムスターに胆管癌が誘発され, ヒトの胆管癌の病理発生を研究するのに適したモデルとして推奨されている.このハムスターモデルでは卵円形細胞oval cellが胆管癌の母細胞と考えられている.もしハムスター由来の純粋な卵円形細胞を多数得ることが出来たら, 胆管癌の組織発生をより直接的に検討できるであろう.本研究で我々は, ハムスターから高純度の卵円形細胞を分離することから出来た.卵円形細胞は, コリン欠乏下でジエチルニトロサミンと2-アセチルアミノフルオレン投与によって誘発され, 遠心洗滌法によって分離された.分離細胞は直径が10.9±1.1μmと大きさが非常に均一で, 核 / 細胞質比が高く, 卵円形の核と少数の細胞質内小器官を有していた.免疫組織化学的には, 分離細胞の85.4±1.6%, 75.1±2.0%, 62.0±1.5%および25.6±2.7%がサイトケラチン19, OV-6, アルブミンあるいはα-胎児性蛋白にそれぞれ陽性であり, 分離細胞は肝細胞と胆管上皮細胞の両方の特徴を持っていた.従って, この分離細胞はハムスターの卵円形細胞であると考えられた.
- 社団法人日本獣医学会の論文
- 2000-03-25
著者
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LEE Yong-Soon
Veterinary Public Health
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Lee Jang-hern
Department Of Veterinary Physiology College Of Veterinary Medicine And School Of Agricultural Biotec
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Kim Doo-young
School Of Electrical Eng. Seoul National Univ.
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Lee Y‐s
Seoul National Univ. Suwon Kor
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Kim D‐y
Seoul National Univ. And School Of Agricultural Biotechnol. Suwon Kor
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Yoon B‐i
Division Of Cell And Molecular Pathogenesis Department Of Pathology Virginia Commonwealth University
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Yoon Byung-il
Virginia Commonwealth University
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Yoon Byung-h
Departments Of Veterinary Pathology Seoul National University
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Yoon Byung-il
Department Of Veterinary Medicine Kangwon National University
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Kim Dae-yong
Department Of Veterinary Pathology College Of Veterinary Medicine Seoul National University And Scho
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Kim Dae-yong
Department Of Veterinary Pathology College Of Veterinary Medicine And Agricultural Biotechnology Seo
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Kim Dae-yong
Departments Of Veterinary Pathology Seoul National University
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Kim Dae-yong
ソウル大学獣医学部獣医病理学教室
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Kim Dae-yong
Department Of Pathology College Of Veterinary Medicine Seoul National University
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Lee Yong-soon
Departments Of Veterinary Public Health College Of Veterinary Medicine And School Of Agricultural Bi
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Lee Yong-soon
Public Health College Of Veterinary Medicine Seoul National University
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Lee Yong-soon
Public Health College Of Veterinary Medicine And School Of Agricultural Biotechnology Seoul National
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Lee Yong-soon
ソウル大学獣医科大学農業生物工学科獣医公衆衛生学講座
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Lee J‐h
Chonnam National Univ. Kor
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Kim D‐y
Department Of Veterinary Pathology College Of Veterinary Medicine Seoul National University And Scho
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Lee John
Laboratories Of Public Hygiene College Of Veterinary Medicine And Bio-safety Research Institute Chon
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Lee You-sang
School Of Electrical Engineering Seoul National University
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LEE Jae-Hyun
Experimental Pathology Laboratory
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JOO Kyung-Whan
Department of Parasitology, College of Medicine, Korea University
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Joo Kyung-whan
Department Of Parasitology College Of Medicine Korea University
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Yoon Byung-il
Division Of Cell And Molecular Pathogenesis Department Of Pathology Virginia Commonwealth University
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