Mycobacterium avium-M. intracellulare Complex の血清学的同定法, 特に螢光抗体法に関する研究

概要

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The fluorescent antibody (FA) test was applied to the serological identification of the Mycobacterium avium-M. intracellulare complex in comparison with the agglutination and complement fixation (CF) test. The results are summarized as follows: (1) As a serological method of identification, CF test was a little less effective than the agglutination test. The agglutinin adsorption was necessary for about half the number of strains of the serotypes examined. A few cross reactions were eliminated by the use of CF antigen purified with chloroform. Complement-fixing substances were detected as an anthronpositive spot by thin-layer chromatography. (2) The indirect fluorescent antibody test (IFAT) was successfully applied to the identification of organisms of the known serotypes, as well as the unknown primary cultures of swine origin. (3) Such cross reactions as observed sometimes in the agglutination and CF tests did not appear in the IFAT, even when unabsorbed immune sera were employed in this test.
社団法人日本獣医学会の論文
1975-02-25