Sphaerophorus necrophorusの凝集反応用抗原の安定性について

概要

論文の詳細を見る
Agglutination reaction may be supposed to be one available method for the serological diagnosis of the infection, and the identification of Sphaerophorus necrophorus, though there are some problems in preparing the stable bacterial suspension as the antigen. Physiological saline suspensions of 20 out of 38 strains spontaneously agglutinated after incubation at 37℃ for 24 hours. Such findings were not altered by lowering the salt concentration of the fluid. During the experiment, the authors gave attention to the fact that the VL basal broth cultured organisms remained stable for a rather long time at room temperature. Based on this finding, several experiments were carried out how to make a good antigen. It became clear that the VL basal broth which contained the normal rabbit sera (1/2, 000), produces the best solution for preparing the antigen of the agglutination reaction. Among the antigens which were prepared from the 38 strains by this method, only 3 showed a slight autoagglutination. Since these could easily be differentiated from the real positive reaction, the antigens prepared by this method can be effectively used for agglutination test.
帯広畜産大学の論文
1974-12-25