ガスクロマトグラフ質量分析計によるラット血清中のGomisin A (TJN-101) 及び代謝物の定量

概要

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Gomisin A (TJN-101) is one of the lignan components isolated from Schisandra Fruits. A high sensitive and precise method for the determination of TJN-101 and its major metabolite (Met. B) in the rat serum was developed by selected ion monitoring (SIM) with gas chromatography-mass spectrometry (GC/MS) using a fused silica capillary column (SPB^<TM>-1,Supelco). A 100 μl serum sample was used for the solid phase extraction. The calibration curves of TJN-101 and Met. B both showed a good linearity between 2.0 and 2000.0 ng/ml. The analytical precision (intra-assay, C.V. <4.7%), recoveries (98.4±10.1%), and detection limit (2 ng/ml) of TJN-101 indicated that this system was suited for the determination of TJN-101 in biological fluid. In case of Met. B, the same results as TJN-101,were obtained. After oral administration of TJN-101 at a dose of 10 mg/kg to male rats, the average values of the maximal serum concentration of TJN-101 and Met. B were 1446.1±131.8 and 317.4±18.5 ng/ml, respectively. The serum concentrations of these substances could be monitored sufficiently for 8 h after dosing.
社団法人日本薬学会の論文
1991-10-25