胆汁酸18種及びコレステロールとその代謝物等4種のキャピラリーGC及びGC/MSにおける挙動

概要

論文の詳細を見る
Hexafluoroisopropyl ester-trifluoroacetate (HFIP-TFA) derivatives or methyl estertrifluoroacetate (Me-TFA) derivatives of 18 bile acids and non-derivatized or trifluoroacetate (TFA) derivatives of 4 neutral steroids containing cholesterol and its metabolites were analyzed by capillary GC. Non-polar or slightly polar columns were effective for the analysis of HFIP-TFA derivatives of bile acids. Non-polar columns were useful for the analysis of Me-TFA derivatives of bile acids. TFA derivatives of 4 neutral steroids were completely separated and analyzed by non-polar columns. Furthermore, HFIP-TFA derivatives of 18 bile acids and TFA derivatives of 4 neutral steroids were analyzed by capillary GC/MS with electron impact (EI) or chemical ionization (CI). Molecular ions ([M]^+ or [MH]^+) did not occasionally appear, but the specific fragment ions ([M-CF_3COOH]^+ or [MH-CF_3COOH]^+ and so on) were observed. Intensities of the specific fragment ions were tabulated.
公益社団法人日本薬学会の論文
1990-10-31