Simultaneous Determination of Oxidized and Reduced Coenzyme Q and α-Tocopherol in Biological Samples by High Performance Liquid Chromatography with Platinum Catalyst Reduction and Electrochemical Detection

概要

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A convenient and sensitive high performance liquid chromatographic method with post-column platinum catalyst reduction and electrochemical detection was applied to the simultaneous determination of oxidized and reduced coenzyme Q (CoQ and CoQH_2) and α-tocopherol (α-TP) in human and rat samples. After the separation of oxidized and reduced CoQ homologues (CoQ_8-CoQ_<10> and CoQ_8H_2-CoQ_<10>H_2) and α-TP on a reversed-phase column, oxidized CoQ homologues were reduced on a platinum catalyst reduction column, and then all the reduced forms were quantified with an electrochemical detector operated in the oxidation mode (+0.6V vs. Ag/AgCl). The order of elution was α-TP, CoQ_8H_2,CoQ_9H_2,CoQ_8,CoQ_<10>H_2,CoQ_9,and CoQ_<10> and all were well separated within 13min. The detection limits at a signal-to-noise ratio of 3 were 20pg for α-TP, 70pg for CoQ_8 and CoQ_8H_2,and 100pg for CoQ_9,CoQ_9H_2,CoQ_<10> and CoQ_<10>H_2. Quantitative recoveries (93-102%) from serum and tissues were obtained for all the analytes studied. This method was applied satisfactorily to the simultaneous determination of CoQ, CoQH_2 and α-TP in biological materials.
社団法人日本薬学会の論文
1994-08-15