ビオチン関連化合物の標識化とその微量分析への利用研究(第3報) : d-ビオチンの還元脱硫によるd-デチオビオチンの選択的トリチウム化について

概要

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d-Dethiobiotin tritiated at selective positions can be prepared by reductive desulfurization of d-biotin with Raney nickel catalyst. Three combinations of the solvent (water) and the catalyst (Raney nickel) were examined as follows : 1) Tritiated water and ordinal Raney nickel (W-2) 2) Tritiated water and ^3H_2 gas-adsorbed Raney nickel 3) Non-labeled water, and Raney nickel adsorbed with tritium gas. Although d-dethiobiotin was labeled with tritium by any of these combinations, labeled products with high specific radioactivity were obtained with ^3H_2-adsorbed Raney nickel in 2) and 3). In order to clarify whether the tritium incorporated into ^3H-d-dethiobiotin is derived from tritium water or ^3H_2-adsorbed catalyst, the tritiation mechanisms were investigated. Tritium atoms were shown to be freely interchangeable between the solvent (^3H_2O) and the catalyst (^3H_2-adsorbed Raney nickel) during the catalytic desulfurization of d-biotin. The tritiated d-dethiobiotin obtained was assumed to be predominantly 5-methyl[^3H]-2-oxo-4-imidazolidinehexanoic [6-methylene-^3H] acid from infrared and nuclear magnetic resonance data of ^2H-d-dethiobiotin prepared by the reductive deuterization of d-biotin using an analogous method.
社団法人日本薬学会の論文
1969-03-25