カラム抽出 : 高速液体クロマトグラフ法による血中フェニトインおよびヒドロキシフェニトインの定量

概要

論文の詳細を見る
A method for the simultaneous determination of phenytoin and it's primary metabolite in humans, 5-(p-hydroxyphenyl)-5-phenylhydantoin, in human serum, was developed by column extraction-high-performance liquid chromatography. Simple and rapid extraction from the serum was carried out by using Extrelut[○!R] pre-packed column and chloroform. The chromatographic procedure utilized a reversed-phase column with 0.01% ammonium acetate in 50% methanol and monitored at 250 nm. The anticonvulsants were eluted at a flow rate of 1.0 ml/min, and their quantities were estimated from their peak height ratios to that of phenacetin added as an internal standard. The method was applied to patient serum samples and compared with a commercially available enzyme immnunoassay kit.
公益社団法人日本薬学会の論文
1980-12-25