Aspergillus nigerによるBiotinの^<35>S標識化の試み

概要

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Labeling of biotin with sulfur-35 was carried out in a medium containing Aspergillus niger with 4.5mCi of H_2^<35>SO_4 and dethiobiotin. A large amount of ^<35>S-amino acid besides ^<35>S-biotin and its related compounds (biotin fraction) were obtained. Following amino acids were identified by a two-dimensional paper chromatography : ^<35>S-Cysteinic acid, ^<35>S-cystine, ^<35>S-homocystine, ^<35>S-methionine, ^<35>S-methionine sulfoxide, and ^<35>S-methionine sulfone. About one-half of the added ^<35>S (46%) remained in the supernatant as non-reacted H_2^<35>SO_4 and or non-hydrolysed proteins, and a greater amount (89%) of radioactvities taken up by the cell were incorporated into ^<35>S-amino acids. In the biotin fraction, 0.7% of the total radioactivity was found. Biotin fraction was isolated as avidin complex, dialysed with ammonium carbonate, and identified chromatographically from radioactivity and by bioassay. From this experiment, biotin was found mainly in an oxidized form such as l-sulfoxide and l-sulfone.
社団法人日本薬学会の論文
1967-04-25