ラット尿中のフェニトイン代謝産物の分析

概要

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Phenytoin (DPH) and its metabolites were examined in rat urine after ^<14>C-DPH administration. High-performance liquid chromatography (HPLC), using LiChrosorb RP-18 column and acetonitrile-methanol-0.4 mM phosphate buffer (pH 6.0) (17 : 28 : 55,v/v) as a mobile phase, was employed for the examination of the metabolites. The eluate from the column was monitored by ultraviolet (UV) detector (250nm), and the radioactivity was measured for each fraction. Radio-thin-layer chromatography and the reverse dilution analysis technique have been introduced in order to obtain more detailed informations on the metabolites. From the results obtained, the HPLC method shows that DPH metabolites were separated from the urine sample without being interfered with the urine components, when monitored by UV detector. It was found that the rat urine after ^<14>C-DPH administration contains some DPH metabolites, such as hydroxyphenytoin, diphenylhydantoic acid, diphenylglycine and also some water-soluble unidentified metabolites.
社団法人日本薬学会の論文
1983-09-25