Kinetic Studies on Pancreatic Lipase Activity in Micellar Systems. III. Effect of Micellar Size
スポンサーリンク
概要
- 論文の詳細を見る
Pancreatic lipase activities toward fatty acid vinyl esters solubilized in micelles of five surfactants, sodium taurodeoxycholate (NaTDC), octa-oxyethylene dodecyl ether (ODE), and three polyoxyethylene nonylphenyl ethers (PNE), were kinetically investigated. The kinetic data for all the micellar systems were well interpreted on the basis of fully competitive inhibition mechanisms, where substrate-free micelles act as an inhibitor. For NaTDC micellar systems, the maximum velocity V, the Michaelis constant K_m, and inhibition constant K_4 were determined with respect to four substrates. The K_m/K_4 values for all the substrates were larger than unity but smaller than those previously obtained for sodium deoxycholate micellar systems. For the micellar systems of ODE and two PNEs with average numbers of oxyethylene units of 10 and 15,the Michaelis plots at constant surfactant concentrations were linear, indicating that the K_m/K_4 value is close to unity. On the other hand, for PNE with an average number of oxyethylene units of 30,a similar plot was concave, which suggests that the K_m/K_4 value is larger than unity. The alteration of the K_m/K_4 value with surfactants was closely correlated with the change in stability of the enzyme-micelle solubilizing substrate complex relative to the enzyme-substrate-free micelle complex ; the relative stability was shown to depend on the micellar size. The maximum velocity was also affected by micellar size.
- 公益社団法人日本薬学会の論文
- 1984-10-25
著者
-
枡井 雅一郎
Faculty Of Pharmaceutical Sciences Osaka University
-
大森 秀信
Faculty of Pharmaceutical Sciences, Osaka University
-
大森 秀信
Faculty Of Pharmaceutical Sciences Osaka University
-
岡田 敏史
国立衛研・大阪
-
Okada S
Division Of Drugs Osaka Branch National Institute Of Health Sciences
-
中原 裕
National Institute of Hygienic Sciences, Osaka Branch
-
岡田 敏史
National Institute of Hygienic Sciences, Osaka Branch
-
枡井 雅一郎
大阪大学薬学部
-
中原 裕
National Institute Of Hygienic Sciences Osaka Branch
-
Okada Satoshi
Division Of Drugs Osaka Branch National Institute Of Health Sciences
関連論文
- Anodic Oxidation of Organophosphorus Compounds. III. Anodic Alkoxylation and Thioalkoxylation of Triphenylphosphine
- Phosphorylation of Alcohols via Anodic P-Halogenation of Dialkyl Hydrogen Phosphites
- 合成基質法による低分子量ヘパリンの抗凝血活性の評価.ファクターXa及びトロンビン活性の測定
- Determination of the Molecular-Weight Distribution of Low-Molecular-Weight Heparins Using High-Performance Gel Permeation Chromatography
- Electrochemical Preparation and Some Reactions of Alkoxy Triphenylphosphonium Ions
- Direct Covalent Modification of Glassy Carbon Surfaces with 1-Alkanols by Electrochemical Oxidation
- Facile Preparation of Allylzinc Species from Allyl Bromides and Unactivated Zinc Induced by a Catalytic Amount of Aluminum Chloride and Their Reactions with Carbonyl Compounds and Acetals
- One-Step Transformation of Carboxylic Acids into Aldehydes Induced by the Electrochemical Oxidation of Ph_3P
- Gold Electrode Modified with a Self-Assembled Monolayer of Thiols as an Electrochemical Detector for Ionic Surfactants
- Allylation of Aldehydes with Allyl Bromides and Zinc Induced by Pyridinium Perchlorates
- VOLTAMMETRIC METHOD AS A TOOL IN DETECTING SURFACTANT-INDUCED DISRUPTION OF A LANGMUIR-BLODGETT FILM
- Reaction of Triphenylphosphine Radical Cation with 1,3-Dicarbonyl Compounds : Electrochemical One-Step Preparation of Dioxomethylenetriphenylphosphoranes
- An Improved Electrochemical Preparation of Alkoxytriphenylphosphonium Salts
- Activation of N-Hydroxy Compounds by P-O Bond Formation through Anodic Oxidation of Triphenylphosphine
- Anodic Phosphonylation of Anthracene
- Electrochemical Oxidation of Triphenylphosphine in the Presence of Allylsilanes : Voltammetric Studies and Regioselective Preparation of Allyltriphenylphosphonium Salts
- Electrochemical Reduction of Tertiary Nitroalkanes to Amines
- シイタケより検出されるホルムアルデヒドに関する研究
- Reaction of Electrochemically Generated Triphenylphosphine Radical Cation with Amides and Ureas
- PHOSPHITE TO PHOSPHATE TRANSFORMATION ELECTROCHEMICALLY CATALYZED BY DISULFIDES
- Electrochemical Transformation of N-Nitrosodialkylamines into N-Nitramines and β-Ketonitrosamines
- ミクロ-マクロ複合電極の試作とそれを用いるボルタンメトリーによるアスコルビン酸共存下でのドーパミンの定量
- 原子吸光分析法による有害性金属の定量(第3報) : 長吸収管を用いた微量鉛の定量
- 原子吸光分析法による有害性金属の定量(第1報) : T字型長吸収管を用いたスズの原子吸光分析
- Preparation of Alkyl Thiocyanates by Electrochemical Oxidation of Thiocyanate Ion in the Presence of Alcohols and Triphenylphosphite
- Voltammetric Behavior of Triphenylphosphine in Acetonitrile at a Glassy Carbon Electrode : A Reinvestigation
- N-NITROSATION OF SECONDARY AMINES EFFECTED BY ELECTROCHEMICAL OXIDATION OF NITRITE ION IN WEAKLY BASIC MEDIA
- One-Step Preparation of 1-Alkenyltriphenylphosphonium Perchlorates by Electrochemical Oxidation of Triphenylphosphine in the Presence of Alkenes(Organic,Chemical)
- Thioalkoxytributyl- and Thioalkoxytriphenylphosphonium Salts : Preparation and Application to the Synthesis of Thiolesters and Unsymmetrical Sulfides(Organic,Chemical)
- Simple Preparation of 3-Oxoalkyltriphenylphosphonium Salts Effected by Using 2,6-Lutidinium Salts(Organic,Chemical)
- Reactivity of Phthalimide-N-oxyl : A Kinetic Study(Organic,Chemical)
- Electrochemical Oxidation of N-Nitrosopiperidines : Dual Pathways for N-Nitramine Formation
- A SIMPLE ELECTROCHEMICAL OXIDATION OF ALDEHYDE ACETALS TO ESTERS IN EUTRAL SOLUTION
- Anodic Oxidation of Amides and Lactams Using N-Hydroxyphthalimide as a Mediator
- Unsymmetrical Anodic C-C Coupling of 2,6-Di-tert-butyl-4-methylphenol
- Synthesis of α- and γ-(N-Carbobenzoxy-and N-tert-Butyloxycarbonyl-L-Glutamyl) cholines
- Electrochemical Oxidation of Olefins Using N-Hydroxyphthalimide as a Mediator
- Electrochemical Oxidation of 2,6-Di-tert-butyl-4-methylphenol in Basic Methanol
- Electrochemical Oxidation of N-Nitrosodialkylamines : Mechanism of N-Nitramine and β-Ketonitrosamine Formation
- Anodic Oxidation of Carboxamides. IV. Anodic Dimerizatin of 4-and 4'-Substituted Benzanilides in the Presence of a Strong Base
- Kinetic Studies on Pancreatic Lipase Activity in Micellar Systems. III. Effect of Micellar Size
- Oxidation of Hydroxylamine Derivatives. VI. Anodic Oxidation of N-Alkyl-β-hydroxyhydroxylamines in Aqueous Buffer Solution
- Effects of Cholesterol on the Miscibility of Synthetic Glucosamine Diesters in Lipid Bilayers and the Entrapment of Superoxide Dismutase into the Positively Charged Liposomes
- Indirect Electroreductive Sequential Radical Reaction Catalyzed by a Ni(II) Complex. One-Step Preparation of Functionalized (Methylene)cyclopentanes
- Indirect Electroreductive Cyclization of N-Allyl and N-Propargylamides Using a Nickel(II) Complex as an Electron-Transfer Catalyst : Selective Formation of Halogenated and Non-halogenated Pyrrolidinones
- Electroreductive Synthesis of Bicyclic Ketones Mediated by Cobalt or Nickel Complexes
- Anodic Oxidation of Amines. VIII. Oxidation of Ethane-1,2-diamines
- ANODIC OXIDATION OF COMPOUNDS HAVING BENZYLIC OR ALLYLIC CARBON AND α-CARBON TO HETERO ATOM USING N-HYDROXYPHTHALIMIDE AS A MEDIATOR
- Acylation of Amines and Alcohols by Anodic Oxidation of N-Phenyl-hydroxamic Acids
- CORRECT MOLECULAR STRUCTURE OF THE PRODUCT OBTAINED FROM THE REACTION OF ACETYLSALICYLOYLCHLORIDE WITH N-PHENYLHYDROXYLAMINE. ELECTROCHEMICAL AND X-RAY CRYSTALLOGRAPHIC STUDIES
- Oxidative Bond Fission of β-Alkanolamines by Chlorine Dioxide and the Effect of an Oxymethylene Group at the β-Carbon on the Reaction
- Oxidation Potential of Hydroxamic Acids and 4-Fluorophenol in the Presence of Added Base. Effect of Hydrogen-bonding
- Anodic Oxidation of Amines. VII. Oxidation of β-Alkanolamines in Aqueous Buffer of pH 10
- Oxidation Potential Shift as a Measure of Hydrogen-Bonding and/or the Basicity
- Anodic Oxidation of Amines. VI. Revised Reaction Scheme for the Oxidation of Ephedrine
- 高分子量ヒアルロン酸とドキシサイクリンからなる含水ゲルの薬物放出挙動
- 注射用デキストランのサイズ排除クロマトグラフィーによる分子量測定と分子量標準品に関する検討(医療と分析化学)
- Pharmaceutical Characterization of Commercially Available Intravenous Fat Emulsions : Estimation of Average Particle Size, Size Distribution and Surface Potential Using Photon Correlation Spectroscopy
- ヒアルロン酸ナトリウムとテトラサイクリン類の相互作用
- キトサンとアルギン酸ナトリウムからなる直打錠の徐放化効果
- トロパンアルカロイドの二相間分配挙動
- アラビン酸塩の電場下での対イオン結合性
- The Role of Solvent in the Partition of Procaine and p-Aminobenzoic Acid between Organic Solvent and Water
- 学問に基づいた四つの分類(薬学領域の分類)
- 支部からの推薦にもっと関与しては
- Mass Spectra of Acyclic Nitrones
- 31 酸化的脱炭酸反応を用いたウロン酸類からアミノサイクリトール類への化学変換
- Reaction of 2,4 (5)-Dialkylimidazoles with Formaldehyde
- Reaction of N-(1-Cyanoalkyl) alkylideneamine N-Oxide with Dipolarophiles and Nucleophiles. III. 1,3-Dipolar Cyclo-addition of N-(1-Cyanoalkyl) alkylideneamine N-Oxide
- A Novel Mannich Reaction Product from 2,4 (5)-Diisopropylimidazole
- Reaction of N-(1-Cyanoalkyl) alkylideneamine N-Oxide with Dipolarophiles and Nucleophiles. II. 1,3-Addition Reaction with Carboxamides
- Reaction of N-(1-Cyanoalkyl) alkylideneamine N-Oxide with Carboxamides
- Anodic Dimerization of Enamines, 2-Cyano-2-phenylvinylamines
- Electron Spin Resonance Spectra of N-Nitrosodialkylamine Radical Cations Electrochemically Generated in Solution
- Anodic Pyridination of 2,6-Di-tert-butyl-4-methylphenol
- Thioureas as Effective Catalysts for N-Nitrosodimethylamine Formation
- Electrolytic Intramolecular Cyclization of N-Alkylcarboxanilides to Benzoxazolium
- Electronic Spectra of α-Cyanobenzylideneanilines
- Kinetic Studies on Pancreatic Lipase Activity in Micellar Systems. II. Effect of Fatty Acid Chain Length of Substrates
- Selective Removal of Protecting Groups for Phosphomonoesters of Nucleotides by Anodic Oxidation
- Reactivity of 2,4 (5)-Dialkylimidazoles. Synthesis of 6,7,8,9-Tetrahydro-5H-imidazo [1,5-a] [1,4] diazepine Derivatives
- Oxidation of N-Alkylhydroxylamines. IV. Anodic Oxidation of N-Alkylhydroxylamines
- N-Nitrosodimethylamine Formation catalyzed by Alkylthioureas. A Kinetic Study
- Anodic Oxidation of Imidate Esters
- Anodic Acetoxylation of 2-Hydroxy-3-methoxy-5-methylbenzaldehyde and Its Schiff Bases
- Anodic Oxidation of Amines. IV. Cyclic Voltammetry and Controlled Potential Electrolysis of 4-Diethylaminoantipyrine in Acetonitrile
- Oxidation of Hydroxylamine Derivatives. IV. Anodic Oxidation of Cycloserine and Its Acetyl Derivatives in Aqueous Buffer Solution
- Oxidation of Hydroxylamine Derivatives. III. Anodic Oxidation of N-Hydoxy and N-Alkoxycarbamates
- C-C Bond Fission in Carboxamide. Intermediacy of Phenylcarbamate Ion in the Alkaline Hydrolysis of Trichloroacetanilide
- Kinetic Studies on the Formation of Dimethylnitrosamine
- Saponin and Sapogenol. XXIX. Selective Cleavage of the Glucuronide Linkage in Oligoglycosides by Anodic Oxidation
- 瀧浦潔先生を偲んで
- 利用者一人一人が提案を(情報化時代を迎えた薬学図書館のあり方)
- Controlled Potential Electrolysis. X. Polarographic Behavior and Electrolytic Reduction Process of Esters of Aliphatic α-Nitrocarboxylic Acids
- Anodic Oxidation of Amines. V. Cyclic Voltammetry and Controlled Potential Electrolysis of Ephedrine and the Related Compounds in Aqueous Buffer Solution
- Oxidation of Hydroxylamine Derivatives. II. Anodic Oxidation of Phenylhydroxylamines
- Oxidation of Hydroxylamine Derivatives. I. Anodic Oxidation of Hydroxamic Acids
- Oxidation of N-Alkylhydroxylamines. V. Anodic Oxidation of N, O-Dialkyl- and N, N, O-Trialkylhydroxylamines
- Oxidation of N-Alkylhydroxylamines. II. Reaction of N-Alkylhydroxylamines with tert-Butylhydroperoxide
- Oxidation of N-Alkylhydroxylamines. I. Nitroxides formed on Oxidation of N-Alkylhydroxylamines with Lead Tetraacetate