Determination of Cytosine, 3-Methylcytosine, and 5-Methylcytosine in Nucleic Acids by High Performance Liquid Chromatography
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概要
- 論文の詳細を見る
A sensitive and selective analytical method for cytosine, 3-methylcytosine, and 5-methylcytosine in nucleic acids by high performance liquid chromatography was developed. This method involves hydrolysis of deoxyribonubleic acid (DNA) or ribonucleic acid (RNA) with perchloric acid at 100°for 1 hr, removal of the acid by weakly basic anion exchange resin (CG-4B, OH-form), separation of cytosine, 3-methylcytosine, and 5-methylcytosine from other bases by treatment with Dowex 1×2 column (HCOO-form), and the analysis of the eluate by high performance liquid chromatography. Recoveries were more than ninety percent. This method was applied to several nucleic acids, and molar ratio of 3-, and 5-methylcytosine to cytosine was obtained.
- 社団法人日本薬学会の論文
- 1977-11-25
著者
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河崎 秀樹
東邦大学薬学部臨床化学教室
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山路 昭
大阪大学医学部附属病院
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山路 昭
Department Of Pharmacy Osaka University Hospital
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由岐 英剛
Faculty of Pharmaceutical Sciences, Osaka University
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由岐 英剛
Faculty Of Pharmaceutical Sciences Osaka University
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由岐 英剛
東邦大学薬学部臨床化学教室
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