サイコ含有漢方エキス製剤中のSaikosaponinの定量

概要

論文の詳細を見る
The quantitative analysis of saikosaponins in Chinese medicinal preparations containing Bupleuri Radix was investigated. Saikosaponins-b_1 and -b_2 in the KOH-MeOH extracts from these preparations were determined by high performance liquid chromatography (HPLC) on LiChrosorb RP-18 column with a mobile phase of MeOH-H_2O-AcOH-Et_3N (75 : 25 : 0.2 : 0.2). Saikosaponins-a and -d were converted quantitatively into the corresponding -b_1 and b_2 by mild acid treatment of the extracts, and determined by HPLC method. This methods were applied to pharmaceutical evaluation of seven kinds of Chinese medicinal preparations containing Bupleuri Radix and other miscellaneous crude drugs. The all preparations contained considerable amounts of saikosaponins-b_1 and -b_2 which were regarded as the secondary products from -a and -d at the manufacturing procedure. Nineteen commercial products of Daisaiko-to and Shosaiko-to contained 33-526 of saikosaponin-a, -281 of -d, 8-449 of -b_1 and 5-335 μg/g of -b_2.
社団法人日本薬学会の論文
1984-07-25