A Simple Fluorescent Post-labeling Technique with o-Phthalaldehyde for the Analysis of Proteins by Polyacrylamide Gel Electrophoresis
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概要
- 論文の詳細を見る
A new polyacrylamide gel electrophoretic system with post-labeling of proteins with o-phthalaldehyde (OPT) has been developed. In the present system, the widely used tris, glycine and ammonium persulfate were replaced by triethanolamine, L-hydroxyproline and sodium persulfate, respectively. Proteins were visualized by dipping the gels in the OPT reagent for several minutes immediately after electrophoresis, and the gels were then scanned in a fluorescence densitometer. Fifty nanograms of bovine serum albumin could be detected by this technique.
- 社団法人日本薬学会の論文
- 1980-12-25
著者
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伊藤 利彦
School Of Pharmaceutical Science Toho University
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由岐 英剛
School of Pharmaceutical Science, Toho University
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矢島 毅彦
School of Pharmaceutical Science, Toho University
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武藤 里志
School of Pharmaceutical Science, Toho University
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武藤 里志
Department Of Clinical Chemistry School Of Pharmaceutical Science Toho University
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由岐 英剛
Department of Clinical Chemistry, School of Pharmaceutical Science, Toho University
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矢島 毅彦
School Of Pharmaceutical Sciences Toho University
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由岐 英剛
Faculty Of Pharmaceutical Sciences Osaka University
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由岐 英剛
Department Of Clinical Chemistry School Of Pharmaceutical Science Toho University
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