Determination of Adriamycin and Its Fluorescent Metabolites in Biological Fluids of Inpatients with Lung Cancer by High Performance Liquid Chromatography

概要

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A normal-phase high performance liquid chromatographic determination of adriamycin and its metabolites, adriamycinol and adriamycinone, in human body fluids (plasma, urine and pleural fluids) is described. Samples were collected from lung cancer patients who had received adriamycin by peripheral vein infusion, bronchial arterial infusion and intrapleural infusion. Adriamycin and its metabolites were easily and efficiently extracted from each biological fluid by the use of an Extrelut^[○!R] column. High performance liquid chromatographic separation was carried out in the presence of Mg^<2+> and the limits of determination of adriamycin, adriamycinone and adriamycinol were found to be 0.52,0.72 and 0.48ng/ml, respectively. Chromatographic analyses were completed within 8min. The present method was also applied to pharmacokinetic studies of adriamycin, and it was found that the metabolic fate of adriamycin can be well explained by a two-compartment model.
社団法人日本薬学会の論文
1985-09-25