Fluorometric Determination of Homopolymeric Peptides with 5-(N, N-Dimethylamino)naphthalene-1-sulfinic Acid after N-Chlorination

概要

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A new fluorometric method of determination for oligopeptides based on the reaction of peptide bonds has been developed. The N-H groups in peptide bonds were chlorinated with tert-butyl hypochlorite, and the resulting N-Cl peptides were reacted with 5-(N, N-dimethylamino)naphthalene-1-sulfinic acid. The product, 5-(N, N-dimethyl-amino)naphthalene-1-sulfonyl chloride, which was formed quantitatively from N-Cl peptides, was extrated with chloroform, and the extract was hydrolyzed to give a highly fluorescent 5-(N, N-dimethylamino)naphthalene-1-sulfonic acid. In the proposed method, the fluorescence intensity obtained from several glycine oligomers (di-, tri-, tetra-, penta- and hexa-glycine) increased in proportion to the concentration of peptides and thus in proportion to the number of peptide bonds. Similar results were obtained with alanine oligomers (di- to hexa-alanine).
公益社団法人日本薬学会の論文
1989-10-25