High-Performance Liquid Chromatographic Determination of Eicosapentaenoic Acid in Serum by a Chemiluminescence Labeling Method

概要

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A sensitive method for the determination of eicosapentaenoic acid (EPA) in human serum and the detection of some other fatty acids was developed. Fatty acids were labeled with N-(4-aminobutyl)-N-ethyl-isoluminol (ABEI), and separated by reversed-phase high-performance liquid chromatography. The eluate was mixed with 0.2 μM microperoxidase solution and 90 mM hydrogen peroxide solution successively, and the chemiluminescence was detected in a flow cell placed in front of a photomultiplier. The calibration curve of EPA was linear in the range of 2 pmol to 2 nmol. The recovery of EPA added to human serum was nearly 100%, and the detection limit was 200 fmol. Eleven fatty acids were detected in human serum samples by this method.
公益社団法人日本薬学会の論文
1988-05-25