過酸化水素系高感度発色試薬の開発研究。水溶性ロフィン誘導体の合成と生体成分分析への適用性の検討

概要

論文の詳細を見る
New hydrogen donors, water soluble lophine derivatives, were synthesized for the determination of hydrogen peroxide developed in the enzymatic reaction of biological substances. These chromogens have high absorptivity ; extinction coefficient is 40 000-50 000 upon oxidation with hydrogen peroxide in the presence of peroxidase. A single work reagent is used for the determination of uric acid in the serum ; the reaction is complete in less than 5 min at 37℃. The blue dye is formed at 640 nm ; a blank sample measurement is not needed. The standard curve for the method is linear for uric acid ranging from 2.5 to 25 mg/dl. Average analytical recovery of uric acid in the human sera exceeded 99% ; within-run and between-run precision studies showed CV's ⪈2.5 and ⪈3.5%, respectively. The method correlated well with the standard uricase-ultraviolet method ; the regression equation is y=0.988x+0.426 (mg/dl) and the correlation coefficient (γ) is 0.977 (n=11).
公益社団法人日本薬学会の論文
1987-11-25