Determination of 7-Methylguanine and 1-Methyladenosine in Urine by High-Performance Liquid Chromatography

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The high-performance liquid chromatographic method for the determination of 7-methylguanine and 1-methyladenosine, the degradation products of ribonudeic acids, in urine was described.The urine samples were at random specimens of normal and cancer patients.One ml of 6N hydrochloric acid was added to 10 ml of the urine.The mixture was filtered through millipore membrane (0.45μm) and an aliquot of the filtrate was injected onto the high-performance liquid chromatograph equipped with cation exchange particle and ultraviolet photometric detector.The separation was performed with phosphate buffer solution (pH 4.5).The peaks of 7-methylguanine, 1-methyladerlosine, and creatinine were successfully detected and determined.when compared to normal control values, the ratio of the base or nucleoside to creatinine increased significantly in the urine of patients.
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