Increased Expression of Hsp70 for Resistance to Deuterium Oxide in a Yeast Mutant Cell Line
スポンサーリンク
概要
- 論文の詳細を見る
Labeling with stable isotopes, typically deuterium (D), is powerful tool for studying the functional structure of biontolecules by NMR. Biosynthesis of certain deuterated proteins in microorganisms cultured in deuterium oxide (D_2O) is an attractive strategy. However, the growth of almost all microorganisms is inhibited at high concentrations of D_2O. We isolated a mutant of yeast that grows well in D_2O. The expression of Hsp70 was enhanced in the mutant. The increased expression also endowed the yeast with cold-resistance. The mutant might be useful for biosynthesis of D-labeled biomolecules.
- 社団法人日本薬学会の論文
- 2003-06-01
著者
-
OKU Naoto
Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka
-
奥 直人
Dep. Of Medical Biochemistry School Of Pharmaceutical Sciences Univ. Of Shizuoka
-
Oku Naoto
静岡県立大学 薬学部
-
Oku N
Univ. Shizuoka Shizuoka Jpn
-
Unno K
Department Of Medical Biochemistry School Of Pharmaceutical Sciences University Of Shizuoka
-
Unno Keiko
静岡県立大学 薬 医薬生命化学
-
Unno Keiko
Department Of Radiobiochemistry School Of Pharmaceutical Sciences University Of Shizuoka
-
Okada Shoji
Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka
-
KISHIDO Takahiro
Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka
-
MORIOKA Mizue
Department of Biological Sciences, Graduate School of Science, University of Tokyo
-
Oku Naoto
Department Of Medical Biochemistry And Coe Program In The 21st Century University Of Shizuoka School
-
Okada S
Department Of Medical Biochemistry School Of Pharmaceutical Sciences University Of Shizuoka
-
Okada S
Department Of Radiobiochemistry School Of Pharmaceutical Sciences University Of Shizuoka
-
Okada S
Tokyo Medical Univ. Tokyo
-
Okada S
Kumamoto Univ. Kumamoto Jpn
-
Okada S
Univ. Shizuoka Shizuoka Jpn
-
Okada Shoji
Department Of Environmental Biochemistry Shizuoka College Of Pharmacy
-
Unno Keiko
Department Of Radiobiochemistry School Of Pharmaceutical Sciences
-
Unno K
Univ. Shizuoka Shizuoka Jpn
-
Morioka Mizue
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
-
Morioka Mizue
Department Of Biological Science Graduate School Of Science The University Of Tokyo
-
奥 直人
静岡県立大学薬学部医薬生命科学教室
-
Kishido Takahiro
Department Of Medical Biochemistry School Of Pharmaceutical Sciences University Of Shizuoka
-
Okada Shoji
Department Of Environmental Biochemistry Shizoka College Of Pharmacy
-
Unno Keiko
Department Of Medical Biochemistry School Of Pharmaceutical Sciences University Of Shizuoka
-
OKU Naoto
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University
-
Okada Shoji
Department of Chemistry, Faculty of Science and Technology, Kinki University
関連論文
- Argonaute2ノックダウンによる腫瘍新生血管傷害療法の開発(誌上シンポジウム)
- DDS製剤開発のためのイメージング技術
- ポリカチオン化ホスファチジン酸コンジュゲートの合成と遺伝子導入キャリヤーとしての機能評価
- Magnetic Resonance Lymphography of Profundus Lymph Nodes with Liposomal Gadolinium-Diethylenetriamine Pentaacetic Acid
- PREPARATION OF RES-AVOIDING LIPOSOMES
- リポソーム化 CNDAC リン脂質誘導体による肺転移癌治療
- CNDACリン脂質誘導体含有RES回避リポソームの抗腫瘍効果
- シアル酸を含有しない糖脂質とインフルエンザA型ウイルスの結合
- Deuteration Causes the Decreased Induction of Heat-Shock Proteins and Increased Sensitivity to Heat Denaturation of Proteins in Chlorella : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- Characteristics of the Photosynthetic Metabolism of Carbon in Deuterated Chlorella ellipsoidea C-27
- Differential Cellular Isotope Effects of Deuterium on Photosynthetic Metabolism of Carbon in Chlorella ellipsoidea
- 第3回国際O-CHA学術会議(ICOS2007)
- Enhanced Experimental Tumor Metastasis with Age in Senescence-Accelerated Mouse(Molecular and Cell Biology)
- DDSを利用した分子イメージングに向けて
- モノカルボン酸系薬物の脂質膜透過機構の解析 - Carrier Mediated like Uptake of Salicylic Acid by Liposomes -
- The Synthesis and in Vitro and in Vivo Stability of 5-Fluorouracil Prodrugs Which Possess Serum Albumin Binding Potency
- In Vitro Efficacy of a Sterically Stabilized Immunoliposomes Targeted to Membrane Type 1 Matrix Metalloproteinase (MT1-MMP)(Biopharmacy)
- Increased Expression of Hsp70 for Resistance to Deuterium Oxide in a Yeast Mutant Cell Line
- Effect of Over-Expressed Hsp26 on Cell Growth of Yeast
- EXPRESSION OF STRESS PROTEIN IN YEAST WHICH IS RESISTANT TO LOW TEMPERATURE AND DEUTERIUM OXIDE
- Role of Hsp70 Subfamily, Ssa, in Protein Folding in Yeast Cells, Seen in Luciferase-Transformed ssa Mutants
- PARTICIPATION OF STRESS PROTEINS IN EXOGENOUS LUCIFERASE EXPRESSION IN YEAST
- Chaperonin-Repairable Subtle Incompleteness of Protein Assembly Induced by a Substitution of Hydrogen with Deuterium : Effect of GroE on Deuterated Ribulose 1,5-Bisphosphate Carboxylase : PROTEINS, ENZYMES AND METABOLISM
- Therapeutic Efficacy of 5-Fluorouracil Prodrugs Using Endogenous Serum Proteins as Drug Carriers : A New Strategy in Drug Delivery System
- Proteomic Characterization of Angiogenic Endothelial Cells Stimulated with Cancer Cell-Conditioned Medium(Molecular and Cell Biology)
- siRNA体内動態のPET解析と新規ベクターの開発
- Argonaute2 (Ago2) 標的siRNA細胞内導入による細胞増殖抑制に関する検討
- 腫瘍新生血管標的化リポソーム (特集 抗がん剤のDrug Delivery System)
- Vulnerability to Seizures Induced by Potassium Dyshomeostasis in the Hippocampus in Aged Rats
- Therapeutic Effect of Reticuloendothelial System (RES)-Avoiding Liposomes Containing a Phospholipid Analogue of 5-Fluorouracil, Dipalmitoylphosphatidylfluorouridine, in Meth A Sarcoma-Bearing Mice
- 薬学共用試験--CBTとOSCE (特集 「参加型」実務実習で次世代を育てる) -- (実習参加学生の資質の確保に向けて)
- 海馬苔状線維-CA3錐体細胞間での長期増強に対する亜鉛の作用
- β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells
- TUMOR ACCUMULATION OF D-SELENOMETHIONINE-^Se IN TUMOR-BEARING MICE
- Alterations in the Heat Response of Chlorella ellipsoidea by Deuteration
- Role of Sialylglycoconjugate(s) in the Initial Phase of Metastasis of Liver-metastatic RAW117 Lymphoma Cells
- Cell-Nuclear Accumulation of 72-kDa Stress Protein Induced by Dimethylated Arsenics
- Cell-nuclear Accumulation of Stress Protein (Hsp72) Induced by Dimethylarsenics (Proceedings of the 20th Symposium on Toxicology and Environmental Health)
- 温度感受性リポソームによる腫瘍壊死因子(TNF)の組織特異的デリバリーのための基礎研究
- RGD構造類似体アルギニルアミノヘキサン酸およびその修飾リポソームによる癌転移抑制
- Growth Delay and Intracellular Changes in Chlorella ellrpsoidea C-27 as a Result of Deuteration : GROWTH AND DEVELOPMENT : STRUCTURE AND FUNCTION OF CELLS
- Suppressed Permeation of Linoleic Acid in a Liposomal Formulation through Reconstructed Skin Tissue(Biopharmacy)
- Skin Whitening Effect of Linoleic Acid Is Enhanced by Liposomal Formulations (Biopharmacy)
- Isolation of a Novel Peptide Homing to Tumor-Derived Neovasculature That Suppresses Tumor Growth
- サポニンによる好中球の活性化
- DNA-Strand Breaks Induced by Dimethylarsinic Acid, a Metabolite of Inorganic Arsenics, Are Strongly Enhanced by Superoxide Anion Radicals
- Molecular Assembly of Zinc-Nickel Hybrid Porphyrin Dimer Using Synthetic 4α-Helix Polypeptides
- 嗅内野刺激に伴う扁桃体外側核からの亜鉛の放出
- リポソーム化 RGD による癌転移抑制
- Functional role of sialyl lewis X and fibronectin-derived RGDS peptide analogue on tumor cell arrest in lungs followed by extravasation.
- Enhancement of Anticancer Activity in Antineovascular Therapy Is Based on the Intratumoral Distribution of the Active Targeting Carrier for Anticancer Drugs(Biopharmacy)
- Influence of Histidine on Zinc Transport into Rat Brain
- Transport and Action of Cadmium and Zinc in Rat Brain(PROCEEDINGS OF 24TH SYMPOSIUM ON TOXICOLOGY AND ENVIRONMENTAL HEALTH)
- Autitumor Activity of 5'-O-Dipalmitoylphosphatidyl 2'-C-Cyano-2'-deoxy-1-β-D-arabino-pentofuranosylcytosine Is Enhanced by Long-Circulating Liposomalization
- Application of Long-Circulating Liposomes to Cancer Photodynamic Therapy
- PETによりがんの転移過程を観察する
- Effect of Freeze-Thawing on Phospholipid/Surfactant Mixed Bilayers
- 転移性癌細胞の生体内動態におけるインテグリンの関与 : PETを用いた解析
- Permeability Change of Liposomal Membrane Induced by Interleukin-1∂
- 高分子デリバリーを目的とした温度感受性リポソームの開発
- Inhibitory Effect of Green Tea Polyphenols on Membrane-Type 1 Matrix Metalloproteinase, MT1-MMP(Analytical Biochemistry)
- PROTON-INDUCED MEMBRANE FUSION OF LIPOSOMES CONTAINING pH-SENSITIVE POLYMER
- Inhibition of Akt (ser473) Phosphorylation and Rapamycin-Resistant Cell Growth by Knockdown of Mammalian Target of Rapamycin with Small Interfering RNA in Vascular Endothelial Growth Factor Receptor-1-Targeting Vector
- Involvement of Metabolic Methylation in Arsenic-Induced Mutagenesis for Mammalian Cells (Proceedings of the 22nd Symposium on Toxicology and Environmental Health)
- DNA Single-Strand Breaks in L-132 Cells Resulting from Inhibition of Repair Polymerization Shortly after Exposure to Dimethylarsinic Acid
- Differential Effect of UV-B and UV-C on DNA Damage in L-132 Cells
- Dimethylarsenics Reveal DNA Damage Induced by Superoxide Anion Radicals
- Mode of Gene Damage Induced by Exposure to UVB and Some Radical Species (Proceedings of the 20th Symposium on Toxicology and Environmental Health)
- Gene Damage in Human Pulmonary Cultured Cells Induced by Dimethylarsenics (Proceedings of the 19th Symposium on Toxicology and Environmental Health)
- Interaction between Dimethylarsenics and Active Oxygen Species in the Induction of Gene Damage (Proceedings of the 19th Symposium on Toxicology and Environmental Health)
- Identification of oligopeptides binding to peritoneal tumors of gastric cancer
- Increased Gene Expression by Cationic Liposomes (TFL-3) in Lung Metastases Following Intravenous Injection(Biopharmacy)
- Anti-angiogenic Activity and Intracellular Distribution of Epigallocatechin-3-gallate Analogs
- Antineovascular therapy with angiogenic vessel-targeted polyethyleneglycol-shielded liposomal DPP-CNDAC
- Chemoprevention of Tumor Metastasis by Liposomal β-Sitosterol Intake(Molecular and Cell Biology)
- Chronopharmacologic Cancer Treatment with an Angiogenic Vessel-Targeted Liposomal Drug(Biopharmacy)
- Antitumor Activity of Liposomal Naphthoquinone Esters Isolated from Thai Medicinal Plant : Rhinacanthus nasutus KURZ.(Biopharmacy)
- Induction of Apoptosis in Tumor Cells by Three Naphthoquinone Esters Isolated from Thai Medicinal Plant : Rhinacanthus nasutus KURZ(Pharmacognosy)
- PEGylation of Liposome Decreases the Susceptibility of Liposomal Drug in Cancer Photodynamic Therapy (Communication to the Editor)
- Cancer Chemotherapy by Liposomal 6-[[2-(Dimethylamino)ethyl]amino]-3-hydroxy-7H-indeno [2,1-c] quinolin-7-one Dihydrochioride (TAS-103), a Novel Anti-cancer Agent(Miscellaneous)
- Potential Usage of Liposomal 4β-Aminoalkyl-4'-0-demethyl-4-desoxypodophyllotoxin (TOP-53) for Cancer Chemotherapy
- Proapoptotic Action of p53-Tom5 in p53-Resistant A549 Human Non-small Cell Lung Cancer Cells through Direct Mitochondrial Dysfunction
- 奨励賞受賞 五十里彰氏の業績
- siRNA実用化の鍵をにぎるDDS
- Antiproliferative naphthoquinone esters from Rhinacanthus nasutus Kurz. roots on various cancer cells
- Hepatocyte Growth Factor and Transforming Growth Factor-β Stimulate both Cell Growth and Migration of Human Gastric Adenocarcinoma Cells^1
- Development of a Simple Cell Invasion Assay System
- Cancer Ant-Angiogenic Therapy
- 初年度の薬学共用試験を終えて
- 発光・蛍光インビボイメージング装置
- Reconstitution of the Na^+/H^+ Antiporter : A New Method for the Determination of H^+ Efflux from Na^+/H^+ Antiporter-Reconstituted Vesicles
- てんかん発作と脳内亜鉛動態
- ラット脳の発育時における亜鉛代謝と学習との関係
- マウス脳内への亜鉛取り込みにおけるヒスチジンの影響
- ラット脳内へのマンガン取り込みにおけるトランスフェリンの役割
- トランスフェリン欠損マウスの脳における鉄の取込
- ラット嗅覚路におけるマンガンの動態と役割
- シナプス神経伝達における亜鉛の役割 : 学習・記憶との関係において
- マンガンのマウス大脳基底核集積機構に関する研究
- 東日本大震災と第131年会(静岡)