Characteristics of the Photosynthetic Metabolism of Carbon in Deuterated Chlorella ellipsoidea C-27

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The photosynthetic metabolism of carbon in fully deuterated cells of Chlorella ellipsoidea C-27 (D-Chlorella), obtained by culture in medium prepared with 100 mol% D_2O, was characterized by examining the activities of several enzymes and the levels of metabolic regulators in a comparison with those of ordinary cells (H-Chlorella). The cellular content of starch in D-Chlorella was more than twice that in H-Chlorella, whereas those of sucrose and glucose were significantly lower in D-Chlorella. Deuteration of Chlorella caused marked alterations in the activities of enzymes involved in starch metabolism. There was a significant decrease in the activity of phosphorylase, a catabolic enzyme, and a significant increase in the activity of starch synthase, an anabolic enzyme. These alterations are probably responsible for the increase in the amount of starch in cells. By contrast, no marked changes were observed in the activities of enzymes and the levels of metabolic inhibitors that are involved in the synthesis of sucrose. It seems likely, therefore, that the decrease in the amount of sucrose in D-Chlorella was caused mainly by a deficiency in sources of carbon in the cytoplasm, as a consequence of an increase in levels of starch in chloroplasts.
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