Alterations in the Heat Response of Chlorella ellipsoidea by Deuteration

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For the elucidation of the isotope effect on cell functions of deuterium (D) incorporated into cell constituents, alterations in the heat response of D-exchanged Chlorella ellipsoidea (D-Chlorella) were investigated. D-Chlorella cells obtained by culture in medium that contained 60 mol% D_2O were assayed for their response to heat in H_2O medium to rule out the solvent isotope effect of D_2O. Upon heating at 41-45℃, the heat sensitivity of D-Chlorella was greater than that of ordinary. (H-Chlorella) cells; at 43℃, the heat sensitivity of D-Chlorella was 1.5-1.6 times higher than that of H-Chlorella. For the induction of resistance to heating, preheating of the cells at a lower temperature than that used for heat treatment was effective in the case of both D- and H-Chlorella. However, the optimum temperature for preheating of D-Chlorella (34℃) was lower than for H-Chlorella (36-37℃). With preheating at 34℃, heat-shock proteins (HSPs), in particular proteins of 62 and 79 kDa, were induced similarly in both types of cell. However, the gel-electrophoretic patterns of HSPs induced at 37℃ were differed somewhat between D- and H-Chlorella. These results suggest that the response of cells to heat, in particular the induction of resistance to heating and the synthesis of HSPs, was altered by deuteration of cell constituents.
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