TUMOR ACCUMULATION OF D-SELENOMETHIONINE-^<75>Se IN TUMOR-BEARING MICE

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The radioactivity distribution of ^<75>Se-labeled D-selenomethionine (D-SeMet-^<75>Se) was investigated in tumor-bearing mice in order to develop a new radioactive diagnostic agent. D-SeMet-^<75>Se was enzymatically prepared from commercially available L-selenomethionine-^<75>Se (L-SeMet-^<75>Se) by using amino acid reacemase and immobilized L-amino acid oxidase. No difference between D- and L-SeMet-^<75>Se was found in the excretion rate into urine and feces in normal mice within 48 h after administration. The in vivo uptake of D-SeMet-^<75>Se in both Ehrlich solid tumor and sarcoma- 180 solid tumor was several times higher than that of L-SeMet-^<75>Se but the uptake of D- and L-SeMet- ^<75>Se were similar in the pancreas. These results indicated that D-SeMet-^<75>Se might be useful as a tumor-imaging agent. In vitro experiments on Ehrlich ascites tumor cells showed that D-SeMet-^<75>Se was incorporated into the tumor cells by a Na^+-dependent active transport system similar to L-SeMet-^<75>Se and that a transport mechanism specific for D-SeMet-^<75>Se might be present in tumor cells in addition to a transport system common to both D- and L-forms.
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