Development of a Simple Cell Invasion Assay System
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概要
- 論文の詳細を見る
Cell invasion assay is important for studying various biological events, such as inflammation, cancer metastasis, and angiogenesis. In this study, we developed a simple method for the quantification of cell invasion by using a culture insert with fluorescence blocking micropore membrane (FBM). Fluorescence labeled cells were simply added to a culture insert with a 8 μm-pore FBM precoated with Matrigel and incubated for an appropriate duration. Then, the FBM was examined under a fluorescence microscope to count the invaded cell number. By this method, accurate invasion assay is easily performed without the steps of fixation and staining of cells and removal of cells which do not invade.
- 公益社団法人日本薬学会の論文
- 2000-10-01
著者
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OKU Naoto
Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka
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Oku Naoto
Department Of Medical Biochemistry And Coe Program In The 21st Century University Of Shizuoka School
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YAMAKAWA Satoru
Department of Medical Biochemistry and COE Program in the 21st Century, University of Shizuoka, Scho
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FURUYAMA Yukihiro
Nippon Becton Dickinson Co., Ltd.,
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Yamakawa Satoru
Department Of Radiobiochemistry School Of Pharmaceutical Sciences University Of Shizuoka
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Furuyama Yukihiro
Nippon Becton Dickinson Co. Ltd.
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OKU Naoto
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University
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