近赤外分光分析法による米麹中の菌体量測定と波長帰属

概要

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Mycelial weight in rice koji was estimated accurately, simply and rapidly by near infrared diffuse reflectance spectrophotometric analysis (NIR) with granular cells. The most accurate calibration was obtained by multiple linear regression analysis using the second derivative (d^2 log (1/R)) of absorbance at four specific wavelengths with 2348 nm as the main one. In this case, multiple correlation of coefficient and standard error of calibration were 0.988 and 0.496,respectively. Prediction values using this calibration curve were not influenced by production year, variety, polishing ratio or shape of the rice used as the raw material. Certain characteristic wavelengths in the NIR spectrum of cultured mycelia, including 2348nm, were observed in the spectra of whole dry koji. Acetone extraction and enzyme lysis of the cell wall of cultured mycelia revealed that absorption at 2348nm was responsible for fat in the mycelia of rice koji.
社団法人日本生物工学会の論文
1995-01-25