細菌アミラーゼに関する研究 : 枯草菌アミラーゼの種類について

概要

論文の詳細を見る
About 100 strains of Bacillus subtilis were studies on their amylases secreted into culture filtrates, and the amylases seemed to be divided into two groups according to the hydrolysis limit on soluble starch ; one hydrolysed about 30〜40%, the other did about 50〜60%. The names of liquefying amylase applied to the former, and saccharifying amylase to the latter, were proposed.Eight strains representing these types were chosen, and the amylases were crystallized by the combination of absorption on starch, treatment with rivanol, and ion exchange chromatography.As the crystalline amylases differed in their stabilities and reaction types not only between two types but also in the same type, these amylases seemed to be different individually.The hydrolysis curves of the crystalline enzyme of a strain on starch was almost identical to that of the crude enzyme, therefore it was assumed that each strain of Bacillus subtilis secreted only one kind of amylase.
公益社団法人日本生物工学会の論文
1963-08-15