Aspergillus nigerのEndo-Polygalacturonaseとそのみかん内果皮剥皮作用

概要

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An enzyme which can remove the segment skin of the mandarin orange (mikan) was sought in wheat bran cultures of about hundred strains of molds, and in several commercial enzyme preparations. It was revealed that several strains belonging to Aspergillus niger and Penicillium produced an enzyme which could remove the segment skin of the mandarin orange. The enzyme was purified from a commercial enzyme preparation (Cellulosin AC, Ueda Chemical Ind. Co.) produced from Aspergillus niger. It was proved that the enzyme was a kind of endo-polygalacturonase and macerated the segment skin of the mandarin orange. Optimum pH and temperature of the enzyme activity on pectic acid were found to be pH 4.2 and 55℃. The enzyme was stable between pH 3 and 8 at 30℃ for 16 hr, but 50% of its activity was lost by standing at 50℃ for 15 min. It was activated and stabilized in the presence of Na^+ or K^+. In the hydrolyzates formed by the segment skin by this enzyme were detected galacturonic acid and unidentified oligosaccharides.
社団法人日本生物工学会の論文
1973-07-25