Penicillium janthinellum BiourgeのXylanaseの精製とその性質 : Hemicellulaseに関する研究(第6報)

概要

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1. Three xylanases (designated as I, II, and III) produced by Penicillim janthinellum Biourge were purified from an extract of wheat bran koji culture by procedures including salting out with ammonium sulfate, SE-Sephadex column chromatography, DEAE-Sephadex column chromatography, and gel filtration on Sephadex G-100. 2. The xylanase I was proved to be homogeneous by ultracentrifugal analysis and three xylanases were practically free from other hemicellulases such as mannanase, galactanase, arabanase, and glycosidases. 3. The xylanase I was most active pH 5.3 and stable over and range of pH 5 to 8 at 30℃ for 24 hr. The xylanase II and III were most active at pH 4.7 and were stable over and range of pH 4 to 9 and pH 5 to 9 at 30℃ for 24 hr, respectively.
社団法人日本生物工学会の論文
1973-07-25