キリ7種の成長点培養

概要

論文の詳細を見る
Searching for the optimum medium for the meristematic culture of Paulownia species, meristematic tips about 0.5 mm long were taken from one to two adult trees (Height : 12〜18m, Diameters at breast height : 22〜38cm) of each of seven species were cultured aseptically on the following four culture media : MURASHIGE and SKOOG medium (MS), broad leaved tree medium (BTM), woody plant medium (WPM), and WOLTER and SKOOG medium (WS). The largest survival rates of the explants were obtained with Paulownia tomentosa, P. glabrata and P. taiwaniana on MS, BTM, and WPM media after 30 days of culture. Smaller survival rates were obtained for P. forturnei and P. coreana on MS and BTM media, and no survival was recorded for P. kawakamii and P. elongata. Larger shoot multiplication rates were recorded for P. tomentosa and P. glabrata on BTM and WPM media after 30 days of subculture on the same media. Judging from the numbers of surviving explants and of shoots developed on the survivors, and by their average shoot lengths, BTM and MS medium are thought to be the best media. The differences of meristematic tips survival rates between species and individuals also were found.
日本森林学会の論文
1989-11-01