Tilia amurensis (アムールシナノキ)の実生幼苗の芽培養による試験管内幼植物体再生とその増殖

概要

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The buds of Ttlla amurensrs RURP seedlings were cultured on the modified MURASHIGE and SKOOG (MS) and SAITO and IDE (IS) media to establish an effective micropropagation method. To investigate the effect of 6-benzylaminopurine (BAP) on shoot development in bud culture, five levels (0,0.5,1.0,2.0,and 5.0 mg/l) of BAP concentrations were tested. The best results were obtained with 1.0 mg/l of BAP in both media. The grand mean of percentages of shoot development for the five levels of BAP in MS medium was somewhat greater than that of IS medium. Developed shoots were subcultured to induce multiple shoots on MS and IS media supplemented with 0.2 mg/l of BAP. The growth of shoots and multiple shoots was stimulated more in explants that were cultured previously on media with 1.0 mg/l of BAP. The maximum rate of multiplication per explant was six times after 30 days culture and the mean was 2.6 times. A 91.7% rooting rate from cultured shoots was achieved on a half-strength MS medium with 0.3 mg/1 of indole-3-butyric acid (IBA) .
日本森林学会の論文
1989-02-01