HPLCによるアミノ酸の高速分析 : PITCによるプレカラム誘導体化の利用

概要

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A rapid quantitative analysis was devised for phenylthiocarbamyl (PTC) derivatives of common amino acids with phenylisothiocyanate (PITC) using reversed-phase HPLC. This study was performed with an HPLC system of Waters Associates. The HPLC conditions were : column, NOVA-PAK cartridge C/18,5×100mm using a Z-module ; column temp., 45±1℃ ; mobile phase, (A) 0.1M ammonium acetate buffer (pH 6.5) containing 5% acetonitrile and (B) 60% acetonitrile in water ; gradient conditions, 0% B to 50% B for 5 min on a linear gradient and 50% B to 100% B for the next 1 min on a linear gradient ; flow-rate, 2.0ml/min ; detection, UV at 254nm ; analysis time, 6 minutes. The retention time had a relative average percent age standard deviation of ±0.39. The correlation coefficients between the peak areas and injected amounts (25 to 500pmol) were more than 0.999. The coefficients of variation in peak areas were 0.68-1.80.
社団法人日本農芸化学会の論文
1987-01-15