Geotrichum candidumが生産する2種類のリパーゼの性質と一次構造

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Geotrichum(G.) candidum produces two extracellular lipases, I and II, lipase I being the predominant component. A lipase I cDNA clone was isolated from a cDNA library by colony hybridization using [ ^<32>P]-labeled oligonucleotides corresponding to a partial amino acid sequence of this enzyme. Subsequently, another lipase II cDNA was isolated by using the lipase I cDNA as a probe. The lipases I and II cDNAs determined by the dideoxy chain termination method coded the N- and C-terminal amino acid sequences and the partial amino acid sequences of the lipases. The mature lipases I and II deduced from the nucleotide sequences were of the same length, 544 amino acid residues. The overall identity between the two lipases was 84%, and there was only a small difference in hydropathy between them. Lipases I and II contained five and four Cys residues, respectively. Four of these residues were completely conserved, and they may particiate in the formation of disulfide bridges. Furthermore, both lipases were glycoproteins, and lipases I and II contained two and three potential N-glycosylation sites, respectively, of which two were conserved. These results suggest that their tertiary structures are quite similar. A homology search indicated that G. candidum lipases and Candida cylindracea lipase were homologous enzymes, and members of the cholinesterase family. A comparison of the primary structure of G. candidum lipases and the enzymes of the cholinesterase family suggested that Ser217 in the sequence of Gly-X-Ser-X-Gly, Asp 104 and His453 or His463 in G. candidum lipases might be the amino acids of the catalytic triad, and that the lipases might form two disulfide bondes between cys61 and cys105,and between Cys276 and Cys288.
社団法人日本生物工学会の論文
1991-09-25

Geotrichum candidumが生産する2種類のリパーゼの性質と一次構造

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