Trichoderma virideのβ-1,3-グルカナーゼの精製と性質

概要

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A β-1,3-glucanase from a solid culture medium of Trichoderma viride was purified by DEAE-Sephadex A-50 and Amberlite CG-50,column chromatographies, and Sephadex G-100 and Bio-Gel P-60 gel filtrations. The molecular weight of the enzyme was estimated to be about 40,000 by the gel filtration method. The maximum activity this enzyme was obtained at about 55℃ and pH 5.0,thermal and pH stable ranges were up to 50℃ and pH 4.0 to 6.0,respectively. The enzyme hydrolyzed laminaritetraose into a large amount of glucose and laminaribiose, and an appreciable amount of laminaritriose. This suggests that this enzyme is the smaller oligosaccharide-producing type of endo-β-1,3-glucanase. Mycolytic enzyme system derived from T. viride produced easily protoplasts from mycelia of Trichoderma reesei QM 9414,Aspergillus awamori var. kawachi, Saccharomyces cerevisiae, and Pleurotus ostreatus (or Pleurotus cornucopiae). Regeneration frequency of the protoplasts formed T. reesei QM 9414,A. awamori var. kawachi and S. cerevisiae were in the range of 10 to 20% of the total protoplasts plated. On the other hand, regeneration frequency of P. ostreatus was less than 1.4%.
社団法人日本生物工学会の論文
1988-09-25