Trichoderma virideのCellulolytic Complexについて : (第5報)AvicelaseとCMCase

概要

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The cellulolytic enzymes derived from Trichoderma viride were obtained from wheat bran Koji and separated into non-adsorbed and adsorbed fractions by the gauze column method. The enzymes in the adsorbed fraction were eluted with distilled water. The enzymes in the non-adsorbed fraction included cellobiase, CMCase and Avicelase activities, but neither filter paper nor gauze degrading activities. These cellulolytic activities were inactivated by heat treatment at 70℃ for 10 min. The enzymes in the adsorbed fraction possessed CMCase and Avicelase activities capable of degrading filter paper and gauze cooperatively, which were thermostable. When both fractions were combined, marked degradation of filter paper and gauze occured, producing much sugar.Remarkable decompositions of Avicel, filter paper and gauze were revealed, when the Avicelase activity in the adsorbed fraction was admixed with the CMCase activity in the same fraction or with CMCase activity in the non-adsorbed fraction.
公益社団法人日本生物工学会の論文
1968-05-25