(1)濾紙崩壊度によるセルレース測定法

概要

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A soluble cellulose derivative, sodium salt of carboxymethyl cellulose, is generally employed as a substrate of cellulolytic enzymes. This substrate, however, is not always adequate for the estimation and characterization of cellulolytic activity. We attempted to establish a simple, ready and accurate assay method using filter paper as substrate. Five milliliters of enzyme solution is taken into L-type glass tube (for Monod's shaking culture apparatus), then two sheets of filter papeer(1×1 cm) are added to the solution. These tubes are shaken in a water bath at 40℃. The filter paper sheets are usually degraded in fine fibers within 150 mim by the extract of wheat bran culture of Trichoderma.An arbitrary cellulase unit can be calculated according to the equation, (150/x.y)×10000,where x is the time in minutes required for the complete breakdown of substrate (disintegration of paper tissue) and y the weight in mg of enzyme preparation in 5 ml solution.Cellulase activity is measured, in ordinary cases, at a 1 percent enzyme concentration, so that the above equation can be simplified as 30000/x. In the present paper, factors affecting the breakdown of filter paper, comparison of several commerical cellulase preparations and the relationship between glucose formation from filter paper and the rate of breakdown are described.
社団法人日本生物工学会の論文
1962-02-15