Trichoderma koningiに依る繊維素分解(第12報)

概要

論文の詳細を見る
It has been shown that there exist in culture fluids after the growth of Trichoderma T610 (koningi type) and T151 (lignorum type) on absorbent cotton cellulase C_l which attacks the native cellulose, such as absorbent cotton, and cellulase C_x which decomposes the shorter or longer chain fragments resulting from the initial attack or chemically modified cellulose, such as filter paper, viscose or cuprammonium rayon and carboxymethylcellulose. The cellulase prepared by the purification procedure using ammonium sulfate and ethanol from the surface culture filtrate of Trichoderma T610 (weak producer of cellobiase) was also able to hydrolyze xylan and possessed no cellobiase activity.The same thermostability of the enzyme after heating at 70℃ for ten minutes could be demonstrated in its action on CMC, filter paper, rayon, cotton and xylan. Therefore, the same enzyme might be the one responsible for the hydrolysis of these substrates.It was observed that this cellulase preparation hydrolyzed filter paper, rayon, cotton with the formatioin of cellobiose and a trace of glucose and hydrolyzed CMC yielding glucose, cellobiose and cellotriose, and that the attack of the cellulase on the xylan chain resulted in the formation of xylose, xylobiose and a mixture of xylooligosaccharides. From those facts it was deduced that they originated from a random attack of the cellulase on the cellulose or xylan chain. The ratio between the products of hydrolysis may vary depending upon the property of the substrate.
公益社団法人日本生物工学会の論文
1959-07-15

Trichoderma koningiに依る繊維素分解(第12報)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク