Trichoderma virideのcellulolytic complexについて : (第3報)濾紙崩壊活性区分の精製

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Four cellulase activities were found in Japan-made commercial cellulase preparations derived from T. viride or Aspergillus niger, namely a gauze degrading activity (C_<1g>), Avicel decomposing activity (C_<1a>), a filter paper degrading activity (C_2) and a CMC decomposing activity (C_3). Cellulase activities in T. viride preparation could be separated into two fractions by using the gauze column method, that is, a C_3 rich fraction and a C_2 rich fraction. C_3 rich fraction passed through the column contains most of C_3,C_<1a> and C_<1g>. Amylase, protease, xylanase, chitinase, pectinase, cell separating enzyme, cellobiase and salicinase were also included in C_3 rich fraction. C_2 rich fraction containing C_2,C_<1a>, C_<1g> and C_3 could be eluted with distilled water without any other enzymic activities.Two components of C_2 were revealed after eluting chromatography with DEAE-Sephadex, A-50 of an enzyme preparation obtained from C_2 rich fraction. Two yellow crystalline C_2-Rivanol complexes could be precipitated by 40 percent saturation with ammonium sulfate following by 70 percent saturation from C_2 rich fraction. Two C_2-Rivanol complexes could be distinguished by electrophoresis using cellulose acetate film as well as an acetone precipitate from C_2 rich fraction. Mixed Rivanol complex was capable of being separated also into two main peaks of C_2 activity after eluting chromatography with DEAE-Sephadex, A-50 column.Another results were as follows; it was mentioned already that C_<1g> was stimulated markedly by adding methylcellulose at an appropriate concentration during incubation with gauze, while other cellulolytic activities were retarded with methylcellulose in the presence of toluene as an antiseptic. In fact, methylcellulose removed an inhibitory effect of toluene on C_<1g>. In our previous papers, it was described that C_3 rich fraction accompanied with little or no C_2 activity degraded various vegetable foodstuffs, though C_2 was considered to be an enzyme mainly attributable to cell wall breakdown. In fact, varying vegetable foodstuffs were degrade into unicells without decomposition of cells walls with C_3 rich fraction. In the case of T. viride preparation, gauze and absorbent cotton were degraded into fine cellulose dust without considerable amounts of weight loss and reducing sugar by shaking incubation and the opposite was shown by stationary incubation. Remarkable adsorption of four cellulase activities with gauze was revealed by shaking incubation.
公益社団法人日本生物工学会の論文
1966-10-25

Trichoderma virideのcellulolytic complexについて : (第3報)濾紙崩壊活性区分の精製

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