Trichoderma koningiのCellulaseとCarboxymethylcellulase

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In the work reported here, particular attention was devoted to pointing out the difference between cellulase and carboxymethylcellulase. The enzyme preparations used were obtained by employing ammonium sulfate (80 per cent saturation) to the extract of wheat bran-sawdust culture of this mold or to the filtrate of a surface culture with synthetic medium containing absorbent cotton as sole carbon source.When both enzyme preparations were subjected to zone electrophoresis using starch as supporting medium in veronal acetic acid buffer at pH 8.5 and at a μ-value of 0.045,only one peak of cellulolytic activity was recognized. On the other hand, separation of the cellulolytic enzymes was accomplished by filter paper electrophoresis under the same conditions starting with the above two enzyme preparations. One component of the cellulolytic enzymes remained in a small portion at the original point, while the other moved on the paper.The former fraction which can easily be adsorbed by filter paper attacked filter paper more rapidly than carboxymethylcellulose, therefore the name of cellulase would preferably be given to this component.Carboxymethylcellulose was more readily decomposed by the latter fraction than filter paper was, so this fraction may be designated as carboxymethylcellulase.These fractions of cellulolytic enzymes were shown to act on all three substrates tested, namely filter paper, carboxymethylcellulose and viscose rayon, but differ in their relative activity from one another. It was also recognized that the proportion of these fractions produced in a culture was varied according to the variety of cellulose preparation added as carbon source.Thus, it was postulated that there were at least two kinds of cellulases produced by the organism employed.
公益社団法人日本生物工学会の論文
1961-05-15

Trichoderma koningiのCellulaseとCarboxymethylcellulase

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