Trichoderma viride の cellulolytic complex について : (第2報) 各種 cellulase 成分とそれらの植物細胞壁分解性

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Cellulase activities in Trichoderma cellulase preparations are capable of being separated into two fractions by using the gauze column method, namely, a fraction rich in CMCase activity and a fraction rich in filter paper degrading activity. Four cellulase activities were found in examining this preparation : a gauze degrading activity (C_<1g>), Avicel decomposing activity (C_<1a>), a filter paper degrading activity (C_2) and a CMCase activity (C_3).It was previously mentioned that C_3 is different from C_2 and both activities may be distinguishable from C_1 C_<1a> and C_<1g> are considered to be cellulolytic components capable of degrading native cellulose. However, C_<1g> was stimulated markedly by the addition of methylcellulose at an appropriate concentration during incubation with gauze, while C<1a> was retarded. Thus it seems that the two components are different from each other.In the case of practical applications of cellulase in food processing it is important to know which cellulolytic component is responsible for the decomposition of the parenchymatous cell wall, as related to the assay method of cellulase activity. So far, from the results obtained it seems that filter paper degrading activity is mainly attributable to cell wall breakdown. In the presence of intercellular substances, such as protopectin, no vegetable foods were degraded by the attack of C_2 activity alone. In the experiment using potato pieces, the substrate was decomposed perfectly into isolated starch with the fraction having maximum filter paper degrading activity in co-operation with a cell separating enzyme. On the other hand, C_3 rich fraction accompanied with little or no C_2 activity decomposed varying vegetable foodstuffs owing mainly to the activities of other enzymes.
公益社団法人日本生物工学会の論文
1965-09-25

Trichoderma viride の cellulolytic complex について : (第2報) 各種 cellulase 成分とそれらの植物細胞壁分解性

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