Trichoderma koningiに依る繊維素分解(第10報)

概要

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The author's intension to get cellulolytic enzymes from the culture of Trichoderma has induced him to set up a liquid medium containing inorganic salts and cotton only.For the production of enzymes surface culture proved to be most satisfactory. Of various strains which mostly secret cellulase, CMCase, amylase, maltase, protcasc and saccharase, only a single one was found to be a weak producer of cellobiase.The properties of the enzymes obtained from the Koji and the culture filtrate of the mold were almost the same in regard to the adequate range of pH values and thermostability, except cellulase and CMCase.The author's effort was concentrated on the separation of cellulolytic enzymes from surface culture. Remarkably enough, both cellulase and CMCase produced in the synthetic medium were thermostable. Heat treatment of the culture fultrate at 70℃ for ten min. followed by precipitation with acetone resulted in obtaining an enzyme preparation which had the activities of cellulase and CMCase exclusively.
公益社団法人日本生物工学会の論文
1958-08-15