Trichoderma virideのCellulolytic complexについて : (第4報) セルラーゼ成分間の相乗効果

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At least four cellulase activities were found in a cellulase preparation derived from T. viride. They were a gauze degrading activity (C_1), Avicel decomposing activity (C_<1a>), a filter paper degrading activity (C_2) and a CMC decomposing activity (C_3).Synergism between C_3 rich and C_2 rich fractions obtained by using the gauze column method, as well as that between two C_2 components revealed in C_2 rich fraction, were investigated. Ferthermore, features in cellulase production ascribable to culture methods, incubation periods and substrates were observed for detection of synergism.1. Gauze was degraded with a cellulase preparation derived from T. viride or Asp. niger, while Avicel was decomposed markedly with a T. viride preparation giving rise to production of cellulose particles passable through filter paper.2. T. viride preparation was separated into C_3 rich and C_2 rich fractions by the author's gauze column method. C_2 activity in C_3 rich fraction was inactivated by heating at 70 C for 20 min. The C_3 activity in C_2 rich fraction was equalized roughly by dilution to that in heattreated C_3 rich fraction. Four cellulase activities in each fraction were compared with each other. Gauze, Avicel and filter paper were decomposed actively with a C_2 rich fraction.3. C_2 activity in T. viride preparation was eliminated by shaking with gauze and by heating at 70 C for 45 min. Synergism was detected in the residual enzyme solution combined with C_2 rich fraction gained by gauze column method in which C_3 activity was equalized to that in the residual enzyme solution. Synergism was shown clearly on C_<1g> and C_2,a little on C_1,and none on C_3.4. Four peaks of C_3 were shown in the eluate when T. viride preparation, from which C_2 activity was removed by shaking with gauze, was fractionated by a DEAE-Sephadex column. Similar synergism was recognized in a C_2 rich fraction accompaning each peak or mixed peaks of C_3 fraction.It was suggested that the C_3 activity contributed not only to reducing sugar production from Avicel, gauze and filter paper, but to the degradation of those substrates.5. Two peaks of C_2 were revealed in the eluate after fractionation using DEAE-Sephadex column of C_2 rich fraction obtained by gauze column method. When two peaks were mixed, synergism was demonstrated on C_<1a>, C_<1g> and C_2 activities but only a little on C_3 activity.6. Features in production of four cellulase components in T. viride wheat bran Koji were investigated. C_3 was detected over the incubation period, even in that case little C_2 was found. Considerable C_<1g> activity was exhibited when C_2 was negligible. Features of C_<1a> production did not coincide with those of C_3 and C_2.Features of C_2 and C_<1g> production resembled each other and both activities were more influenced with C_3 activity than in C_<1a> activity.7. Distinct features were observed in submerged production of T. viride four cellulase components as contrasted with the Koji process.
公益社団法人日本生物工学会の論文
1967-07-25

Trichoderma virideのCellulolytic complexについて : (第4報) セルラーゼ成分間の相乗効果

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