11) 市販セルレース製剤による天然セルロースの分解について

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Commerical cellulase preparations derived from molds, such as Trichoderma viride and Aspergillus niger, are being manufactured in Japan and U.S.A. Their cellulolytic activities are measured with CMC as a substrate. The authors have previously reported on an assay method for cellulase on the breakdown of filter paper.Both substrates, however, are considered as modified celluloses, so in this study we have attempted to determine cellulase C_1 activity by the use cotton thread as native cellulose. Five milliliters of enzyme solution (pH 5.0) were placed in an L-type glass tube for Monod's shaking culture apparatus. Cotton sewing machine thread(three twisted threads, weight 0.0385g/m, count 15,30 twist/cm ranged from 26 to 34 twist) was suspended from a cork plug and added to the solution. An imitation pearl, weight 0.361g, was tied to the endo of the cotton thread as a weight. These tubes were shaked in a water bath at 40℃, at 72rpm. The cotton thread was usually cut within 120 min by the extract of wheat bran culture of a strain of T. vitide(=T. koningi). An arbitracy cellulase unit can be calculated according to the equation, (120/XY)・10000,where X is the time in min required for the breakdown of substrate and Y the weight in mg og enzyme preparation in 5ml solution. Cellulase C_1 activity is measured, in ordinary cases, at a 1 percent enzyme concentration, so that the above equation can be simplified as 24000/X.In the present paper, factors affecting the breakdown of cotton thread, comparison of several commerical cellulase preparations and reducing sugar formation accompanied with the weight-loss of gauze or gauze inoculated with T. viride when treated with cellulase solutions derived from T. viride on a rotary shaker at pH 4.0-5.0,room temprature for 24hr are descrived. It was suggested that cellulase C_1 acts on native cellulose so as to form fine disintegrated fibers rather than to decrease its weight and to produce reducing sugar from such substrate.Furthermore, the authors studied the differences which may exist amoung C_1(cellulase hydrolysing native cellulose, such as cotton thread), C_2 (cellulase hydrolysing modified insoluble cellulose, such as filter paper and swollen cellulose) and C_8(cellulase hydrolysing modified soluble cellulose, such as CMC).
公益社団法人日本生物工学会の論文
1963-05-15

11) 市販セルレース製剤による天然セルロースの分解について

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